CRHR1 antibody (AA 107-117)

Catalog No. ABIN238557

Product Details anti-CRHR1 Antibody

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Binding Specificity: AA 107-117

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: CRHR1 / CRF-R (aa 107 - 117)

Sequence: NEEKKSKVHY H

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with di

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the membranes, vesicles and cytoplasm of MCF7 and Neuro2a cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CRHR1 antibody (ABIN238557)

Xie, Penzes, Srivastava: "Exchange protein directly activated by cAMP 2 is required for corticotropin-releasing hormone-mediated spine loss." in: The European journal of neuroscience, Vol. 50, Issue 7, pp. 3108-3114, (2020) (PubMed).

Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).

Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRHR1 (CRHR1 Products)

Background: CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor

Gene ID: 1394, 12921, 58959

NCBI Accession: NP_004373

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity