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MITF antibody (Microphthalmia-Associated Transcription Factor) Primary Antibody

MITF Reactivity: Human, Mouse, Rat EMSA, IHC (fro), IP, WB Host: Mouse Monoclonal C5 unconjugated
Pubmed (4)
Catalog No. ABIN264430
$919.60
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    MITF
    Reactivity
    • 158
    • 71
    • 43
    • 30
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Human, Mouse, Rat
    Host
    • 95
    • 62
    • 1
    • 1
    Mouse
    Clonality
    • 87
    • 72
    Monoclonal
    Conjugate
    • 77
    • 9
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This MITF antibody is un-conjugated
    Application
    • 74
    • 69
    • 65
    • 58
    • 38
    • 24
    • 13
    • 12
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Electrophoretic Mobility-Shift Assay (EMSA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    Recognizes in Western blotting a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.does not
    Characteristics
    Synonyms: Microphthalmia-associated transcription factor, Mi-protein
    Purification
    Purified
    Immunogen
    Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with anN-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. Genename: MITF
    Clone
    C5
    Isotype
    IgG1
  • Application Notes
    Gel Supershift. Western Blot (1 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry (Frozen and Paraffin Sections). Positive Control: 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclastcells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS containing 0.08 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: One year from despatch.
    Expiry Date
    12 months
  • Gehlsen, Szaszák, Gebert, Koop, Hüttmann, Steven: "Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells." in: Journal of biophotonics, Vol. 8, Issue 6, pp. 466-79, 2016 (PubMed).

    Hägglund, Berghard, Carlsson: "Canonical Wnt/?-catenin signalling is essential for optic cup formation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81158, 2013 (PubMed).

    Yamada, Ohno, Aoki, Semi, Watanabe, Moritake, Shiozawa, Kunisada, Kobayashi, Toguchida, Shimizu, Hara, Yamada: "EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 600-10, 2013 (PubMed).

    Nakano, Ando, Takata, Kawada, Muguruma, Sekiguchi, Saito, Yonemura, Eiraku, Sasai: "Self-formation of optic cups and storable stratified neural retina from human ESCs." in: Cell stem cell, Vol. 10, Issue 6, pp. 771-85, 2012 (PubMed).

  • Target
    MITF
    Alternative Name
    MITF (MITF Antibody Abstract)
    Synonyms
    MITF, ws2a, mitfa, MITF-A, LOC100313655, mitfb, CMM8, MI, WS2, WS2A, bHLHe32, BCC2, Bhlhe32, Gsfbcc2, Vitiligo, Wh, bw, mi, vit, MITF-H, MITF-M, CMI9, melanogenesis associated transcription factor, microphthalmia-associated transcription factor, microphthalmia-associated transcription factor S homeolog, Microphthalmia, MITF, mitf, LOC100313655, mitf.S, LOC100534525, MICROP, Mitf
    Background
    There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3Synonyms: Mi-protein, Microphthalmia-associated transcription factor
    Molecular Weight
    52-56 kDa
    Gene ID
    4286
    UniProt
    O75030
    Pathways
    Chromatin Binding
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