MITF antibody (Ser73, Ser180)

Catalog No. ABIN7180048

Product Details anti-MITF Antibody

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Binding Specificity: Ser73, Ser180

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human, Mouse

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF (MITF Products)

Synonyms: MITF antibody, ws2a antibody, mitfa antibody, MITF-A antibody, LOC100313655 antibody, mitfb antibody, CMM8 antibody, MI antibody, WS2 antibody, WS2A antibody, bHLHe32 antibody, BCC2 antibody, Bhlhe32 antibody, Gsfbcc2 antibody, Vitiligo antibody, Wh antibody, bw antibody, mi antibody, vit antibody, MITF-H antibody, MITF-M antibody, CMI9 antibody, melanogenesis associated transcription factor antibody, microphthalmia-associated transcription factor antibody, microphthalmia-associated transcription factor S homeolog antibody, Microphthalmia antibody, MITF antibody, mitf antibody, LOC100313655 antibody, mitf.S antibody, LOC100534525 antibody, MICROP antibody, Mitf antibody

Background:

Background: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

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Aliases: BHLHE32 antibody, bHLHe32 antibody, Class E basic helix-loop-helix protein 32 antibody, CMM8 antibody, Homolog of mouse microphthalmia antibody, Mi antibody, Microphthalmia associated transcription factor antibody, Microphthalmia, mouse, homolog of antibody, Microphthalmia-associated transcription factor antibody, MITF antibody, MITF_HUMAN antibody, mitfa antibody, nacre antibody, WS2 antibody, WS2A antibody, z3A.1 antibody

UniProt: O75030

Pathways: Chromatin Binding