EZH2 antibody (AA 353-451)

Catalog No. ABIN2668955

Product Details anti-EZH2 Antibody

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Binding Specificity: AA 353-451

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Chromatin Immunoprecipitation (ChIP)

Purification: Protein G Chromatography

Immunogen: This EZH2 antibody was raised against a recombinant protein corresponding to amino acids 353-451 of human EZH2.

Clone: AC22

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-EZH2 antibody (ABIN2668955)

Toth, Papp, Brulois, Choi, Gao, Jung: "LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection." in: PLoS pathogens, Vol. 12, Issue 9, pp. e1005878, (2016) (PubMed).

Ranzani, Rossetti, Panzeri, Arrigoni, Bonnal, Curti, Gruarin, Provasi, Sugliano, Marconi, De Francesco, Geginat, Bodega, Abrignani, Pagani: "The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4." in: Nature immunology, Vol. 16, Issue 3, pp. 318-325, (2015) (PubMed).

Bengani, Mendiratta, Maini, Vasanthi, Sultana, Ghasemi, Ahluwalia, Ramachandran, Mishra, Brahmachari: "Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome." in: PLoS ONE, Vol. 8, Issue 6, pp. e67217, (2013) (PubMed).

Wang, Pan, Lee, Martinez, Feng, Dong: "Cutting edge: Smad2 and Smad4 regulate TGF-β-mediated Il9 gene expression via EZH2 displacement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 10, pp. 4908-12, (2013) (PubMed).

Rossetto, Tarrant-Elorza, Pari: "Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells." in: PLoS pathogens, Vol. 9, Issue 5, pp. e1003366, (2013) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." in: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." in: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Murata, Kondo, Sugimoto, Kawashima, Saito, Isomura, Kanda, Tsurumi: "Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells." in: Journal of virology, Vol. 86, Issue 9, pp. 4752-61, (2012) (PubMed).

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2 (EZH2 Products)

Molecular Weight: 96 kDa

Gene ID: 2146

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation