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5-Hydroxymethylcytosine (5-hmC) antibody Primary Antibody

5-hmC DB, hmeDIP, ICC, IF Host: Mouse Monoclonal 59-1
Pubmed (3)
Catalog No. ABIN2668979
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100 μg
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  • Target
    Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Protein G Chromatography
    This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    For Research Use only
  • Concentration
    1 μg/μL
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    -20 °C
    Storage Comment
    Antibodies in solution can be stored at -20 °C for 2 years.
    Expiry Date
    6 months
  • Jia, Li, Cong, Yang, Sun, Parvizi, Zhao: "Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter." in: PLoS ONE, Vol. 8, Issue 5, pp. e63855, 2013 (PubMed).

    Li, ONeill: "5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote." in: PLoS ONE, Vol. 8, Issue 5, pp. e63689, 2013 (PubMed).

    Li, Zou, Jia, Zhao: "Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets." in: BMC veterinary research, Vol. 9, pp. 87, 2013 (PubMed).

  • Target
    Alternative Name
    Target Type
    In addition to this monoclonal, Active Motif offers two polyclonal antibodies that recognize 5- hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal (39791). The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 μL per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
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