5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668979

Cited in 3+ publications. The Mouse Monoclonal anti-5-Hydroxymethylcytosine antibody (Clone 59-1) (ABIN2668979) specifically detects 5-Hydroxymethylcytosine in DB, MeDIP, IHC, IF and ICC.

Quick Overview for 5-Hydroxymethylcytosine antibody (ABIN2668979)

Target: 5-Hydroxymethylcytosine (5-hmC)

Host: Mouse

Clonality: Monoclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 59-1

Product Details anti-5-Hydroxymethylcytosine Antibody

Purpose: 5-Hydroxymethylcytosine (5-hmC) antibody (mAb) (Clone 59.1)

Purification: Protein G Chromatography

Immunogen: This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Isotype: IgG2a

Application Details

Application Notes: MeDIP: 1 - 2 μg per IP MeDIP-chip: 10 μg per IP DB: 0.2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668979)

Jia, Li, Cong, Yang, Sun, Parvizi, Zhao: "Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter." in: PLoS ONE, Vol. 8, Issue 5, pp. e63855, (2013) (PubMed).

Li, ONeill: "5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote." in: PLoS ONE, Vol. 8, Issue 5, pp. e63689, (2013) (PubMed).

Li, Zou, Jia, Zhao: "Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets." in: BMC veterinary research, Vol. 9, pp. 87, (2013) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine

Target Type: Chemical

Background: In addition to this monoclonal, Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal (39791). The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 µL per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.