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5-Hydroxymethylcytosine (5-hmC) antibody Primary Antibody

5-hmC DB, FACS, hmeDIP, ICC, IF, IHC Host: Rabbit Polyclonal
Pubmed (52)
Catalog No. ABIN2668981
$519.17
Plus shipping costs $45.00 and $24.00 dry ice
100 μL
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Dot Blot (DB), Flow Cytometry (FACS), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Purification
    None
    Immunogen
    This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage
    -20 °C
    Storage Comment
    Antibodies in solution can be stored at -20 °C for 2 years.
  • Zhong, Wang, Li, Zhang, An, Hou: "Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro." in: Scientific reports, Vol. 7, pp. 43539, 2017 (PubMed).

    Page, Paoli, Moran Salvador, White, French, Mann: "Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape." in: Journal of hepatology, Vol. 64, Issue 3, pp. 661-73, 2016 (PubMed).

    Pegoraro, Bafna, Davies, Shuker, Tauber: "DNA methylation changes induced by long and short photoperiods in Nasonia." in: Genome research, Vol. 26, Issue 2, pp. 203-10, 2016 (PubMed).

    Marina, Sturgill, Bailly, Thenoz, Varma, Prigge, Nanan, Shukla, Haque, Oberdoerffer: "TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing." in: The EMBO journal, Vol. 35, Issue 3, pp. 335-55, 2016 (PubMed).

    Brasa, Mueller, Jacquemont, Hahne, Rozenberg, Peters, He, McCormack, Gasparini, Chibout, Grenet, Moggs, Gomez-Mancilla, Terranova: "Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome." in: Clinical epigenetics, Vol. 8, pp. 15, 2016 (PubMed).

    Shi, Yu, Luo, Zhang, Shi, Feng, Chen, He: "Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153100, 2016 (PubMed).

    Nestor, Ottaviano, Reinhardt, Cruickshanks, Mjoseng, McPherson, Lentini, Thomson, Dunican, Pennings, Anderton, Benson, Meehan: "Rapid reprogramming of epigenetic and transcriptional profiles in mammalian culture systems." in: Genome biology, Vol. 16, pp. 11, 2015 (PubMed).

    Vieweg, Dvorakova-Hortova, Dudkova, Waliszewski, Otte, Oels, Hajimohammad, Turley, Schorsch, Schuppe, Weidner, Steger, Paradowska-Dogan: "Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 31, 2015 (PubMed).

    Her, Nelson-Holte, Maher: "Oxygen concentration controls epigenetic effects in models of familial paraganglioma." in: PLoS ONE, Vol. 10, Issue 5, pp. e0127471, 2015 (PubMed).

    Thomson, Fawkes, Ottaviano, Hunter, Shukla, Mjoseng, Clark, Coutts, Murphy, Meehan: "DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures." in: Scientific reports, Vol. 5, pp. 9778, 2015 (PubMed).

    Gustafson, Yang, Dickson, Shao, Van Booven, Harbour, Liu, Wang: "Epigenetic reprogramming of melanoma cells by vitamin C treatment." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 51, 2015 (PubMed).

    Wang, Sun, Jiao, Chong, Chen, Wang, Zhao, Zhou, Li: "Genome-wide 5-hydroxymethylcytosine modification pattern is a novel epigenetic feature of globozoospermia." in: Oncotarget, Vol. 6, Issue 9, pp. 6535-43, 2015 (PubMed).

    Ma, Jiang, Deng, Gu, Wu, Li, Xia, Yang, Ye, Xiong, Guan: "D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth." in: Oncotarget, Vol. 6, Issue 11, pp. 8606-20, 2015 (PubMed).

    Irie, Weinberger, Tang, Kobayashi, Viukov, Manor, Dietmann, Hanna, Surani: "SOX17 is a critical specifier of human primordial germ cell fate." in: Cell, Vol. 160, Issue 1-2, pp. 253-68, 2015 (PubMed).

    Feng, Wang, Li, Zeng, Kuang, Li, Yue: "TET1-mediated different transcriptional regulation in prostate cancer." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 203-11, 2015 (PubMed).

    Jin, Lu, Jelinek, Liang, Estecio, Barton, Issa: "TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells." in: Nucleic acids research, Vol. 42, Issue 11, pp. 6956-71, 2014 (PubMed).

    Wu, Brenner: "Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation." in: Cell reports, Vol. 9, Issue 5, pp. 1827-40, 2014 (PubMed).

    Ahsan, Raabe, Haffner, Vaghasia, Warren, Quezado, Ballester, Nazarian, Eberhart, Rodriguez: "Increased 5-hydroxymethylcytosine and decreased 5-methylcytosine are indicators of global epigenetic dysregulation in diffuse intrinsic pontine glioma." in: Acta neuropathologica communications, Vol. 2, pp. 59, 2014 (PubMed).

    Thomson, Lempiäinen, Hackett, Nestor, Müller, Bolognani, Oakeley, Schübeler, Terranova, Reinhardt, Moggs, Meehan: "Non-genotoxic carcinogen exposure induces defined changes in the 5-hydroxymethylome." in: Genome biology, Vol. 13, Issue 10, pp. R93, 2014 (PubMed).

    Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, 2014 (PubMed).

  • Target
    Alternative Name
    5-Hydroxymethylcytosine
    Target Type
    Chemical
    Background
    Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 μL per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010),the purified IgG version (39791) is likely to work in IF as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
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