5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668981

There are 52+ publications for this product available. The Rabbit Polyclonal anti-5-Hydroxymethylcytosine antibody is suitable to detect 5-Hydroxymethylcytosine. It has been validated for DB, MeDIP, IHC, IF, ICC and FACS.

Quick Overview for 5-Hydroxymethylcytosine antibody (ABIN2668981)

Target: 5-Hydroxymethylcytosine (5-hmC)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-5-Hydroxymethylcytosine Antibody

Purpose: 5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

Purification: Unpurified

Immunogen: This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Application Details

Application Notes: MeDIP: 0.1 - 0.5 µL per IP DB: 1:10,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668981)

Zhong, Wang, Li, Zhang, An, Hou: "Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro." in: Scientific reports, Vol. 7, pp. 43539, (2017) (PubMed).

Page, Paoli, Moran Salvador, White, French, Mann: "Hepatic stellate cell transdifferentiation involves genome-wide remodeling of the DNA methylation landscape." in: Journal of hepatology, Vol. 64, Issue 3, pp. 661-73, (2016) (PubMed).

Pegoraro, Bafna, Davies, Shuker, Tauber: "DNA methylation changes induced by long and short photoperiods in Nasonia." in: Genome research, Vol. 26, Issue 2, pp. 203-10, (2016) (PubMed).

Marina, Sturgill, Bailly, Thenoz, Varma, Prigge, Nanan, Shukla, Haque, Oberdoerffer: "TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing." in: The EMBO journal, Vol. 35, Issue 3, pp. 335-55, (2016) (PubMed).

Brasa, Mueller, Jacquemont, Hahne, Rozenberg, Peters, He, McCormack, Gasparini, Chibout, Grenet, Moggs, Gomez-Mancilla, Terranova: "Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome." in: Clinical epigenetics, Vol. 8, pp. 15, (2016) (PubMed).

Shi, Yu, Luo, Zhang, Shi, Feng, Chen, He: "Loss of 5-Hydroxymethylcytosine Is an Independent Unfavorable Prognostic Factor for Esophageal Squamous Cell Carcinoma." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153100, (2016) (PubMed).

Nestor, Ottaviano, Reinhardt, Cruickshanks, Mjoseng, McPherson, Lentini, Thomson, Dunican, Pennings, Anderton, Benson, Meehan: "Rapid reprogramming of epigenetic and transcriptional profiles in mammalian culture systems." in: Genome biology, Vol. 16, pp. 11, (2015) (PubMed).

Vieweg, Dvorakova-Hortova, Dudkova, Waliszewski, Otte, Oels, Hajimohammad, Turley, Schorsch, Schuppe, Weidner, Steger, Paradowska-Dogan: "Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 31, (2015) (PubMed).

Her, Nelson-Holte, Maher: "Oxygen concentration controls epigenetic effects in models of familial paraganglioma." in: PLoS ONE, Vol. 10, Issue 5, pp. e0127471, (2015) (PubMed).

Thomson, Fawkes, Ottaviano, Hunter, Shukla, Mjoseng, Clark, Coutts, Murphy, Meehan: "DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures." in: Scientific reports, Vol. 5, pp. 9778, (2015) (PubMed).

Gustafson, Yang, Dickson, Shao, Van Booven, Harbour, Liu, Wang: "Epigenetic reprogramming of melanoma cells by vitamin C treatment." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 51, (2015) (PubMed).

Wang, Sun, Jiao, Chong, Chen, Wang, Zhao, Zhou, Li: "Genome-wide 5-hydroxymethylcytosine modification pattern is a novel epigenetic feature of globozoospermia." in: Oncotarget, Vol. 6, Issue 9, pp. 6535-43, (2015) (PubMed).

Ma, Jiang, Deng, Gu, Wu, Li, Xia, Yang, Ye, Xiong, Guan: "D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth." in: Oncotarget, Vol. 6, Issue 11, pp. 8606-20, (2015) (PubMed).

Irie, Weinberger, Tang, Kobayashi, Viukov, Manor, Dietmann, Hanna, Surani: "SOX17 is a critical specifier of human primordial germ cell fate." in: Cell, Vol. 160, Issue 1-2, pp. 253-68, (2015) (PubMed).

Feng, Wang, Li, Zeng, Kuang, Li, Yue: "TET1-mediated different transcriptional regulation in prostate cancer." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 203-11, (2015) (PubMed).

Jin, Lu, Jelinek, Liang, Estecio, Barton, Issa: "TET1 is a maintenance DNA demethylase that prevents methylation spreading in differentiated cells." in: Nucleic acids research, Vol. 42, Issue 11, pp. 6956-71, (2014) (PubMed).

Wu, Brenner: "Suppression of TET1-dependent DNA demethylation is essential for KRAS-mediated transformation." in: Cell reports, Vol. 9, Issue 5, pp. 1827-40, (2014) (PubMed).

Ahsan, Raabe, Haffner, Vaghasia, Warren, Quezado, Ballester, Nazarian, Eberhart, Rodriguez: "Increased 5-hydroxymethylcytosine and decreased 5-methylcytosine are indicators of global epigenetic dysregulation in diffuse intrinsic pontine glioma." in: Acta neuropathologica communications, Vol. 2, pp. 59, (2014) (PubMed).

Thomson, Lempiäinen, Hackett, Nestor, Müller, Bolognani, Oakeley, Schübeler, Terranova, Reinhardt, Moggs, Meehan: "Non-genotoxic carcinogen exposure induces defined changes in the 5-hydroxymethylome." in: Genome biology, Vol. 13, Issue 10, pp. R93, (2014) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine

Target Type: Chemical

Background: Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 µL per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010),the purified IgG version (39791) is likely to work in IF as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.