5-Methylcytosine antibody Primary Antibody
DB, MeDIP, MeDIP-Seq
Host: Mouse
Monoclonal
A1
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Catalog No. ABIN2668983
$519.17
Plus shipping costs $45.00 and $24.00 dry ice
100 μg
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United States
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- Target
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Un-conjugated
- Application
- Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP), Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq)
- Purification
- Protein A Chromatography
- Immunogen
- This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
- Clone
- A1
- Isotype
- IgG1
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-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Concentration
- 1 μg/μL
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -20 °C
- Storage Comment
- Antibodies in solution can be stored at -20 °C for 2 years.
- Expiry Date
- 6 months
-
- Target
- Target Type
- Chemical
- Background
- 5-Methylcytosine (5-mC) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone A1) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
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