5-Methylcytosine antibody

Catalog No. ABIN2668982

Product Details

Target: 5-Methylcytosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Methylated DNA Immunoprecipitation (MeDIP), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (IHC)

Purification: Protein A Chromatography

Immunogen: Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.

Clone: 33D3

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 10 mM phosphate, 0.15 M NaCl and 0.01 % thimerosal. Thimerosal is highly toxic.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References

Borghesan, Fusilli, Rappa, Panebianco, Rizzo, Oben, Mazzoccoli, Faulkes, Pata, Agodi, Rezaee, Minogue, Warren, Peterson, Sedivy, Douet, Buschbeck, Cappello, Mazza, Pazienza, Vinciguerra: "DNA Hypomethylation and Histone Variant macroH2A1 Synergistically Attenuate Chemotherapy-Induced Senescence to Promote Hepatocellular Carcinoma Progression." in: Cancer research, Vol. 76, Issue 3, pp. 594-606, (2016) (PubMed).

McPherson, Fullston, Kang, Sandeman, Corbett, Owens, Lane: "Paternal under-nutrition programs metabolic syndrome in offspring which can be reversed by antioxidant/vitamin food fortification in fathers." in: Scientific reports, Vol. 6, pp. 27010, (2016) (PubMed).

Banh, Iorio, Marcotte, Xu, Cojocari, Rahman, Pawling, Zhang, Sinha, Rose, Isasa, Zhang, Wu, Virtanen, Hitomi, Habu, Sidhu, Koizumi, Wilkins, Kislinger, Gygi, Schofield, Dennis, Wouters, Neel: "PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia." in: Nature cell biology, Vol. 18, Issue 7, pp. 803-813, (2016) (PubMed).

Harrison, Cornett, Goldfarb, DaRosa, Li, Yan, Dickson, Guo, Cantu, Kaustov, Brown, Arrowsmith, Erie, Major, Klevit, Krajewski, Kuhlman, Strahl, Rothbart: "Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1." in: eLife, Vol. 5, (2016) (PubMed).

Yang, Ma, Li, Zhang, Wan, Du, Li, Yang, Lu: "Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression." in: Oncology letters, Vol. 12, Issue 4, pp. 2789-2795, (2016) (PubMed).

Ivanov, Kals, Kacevska, Barragan, Kasuga, Rane, Metspalu, Milani, Ingelman-Sundberg: "Ontogeny, distribution and potential roles of 5-hydroxymethylcytosine in human liver function." in: Genome biology, Vol. 14, Issue 8, pp. R83, (2014) (PubMed).

Xu, Wang, Long, Zhang, Wei, Xing, Gu, Xie: "Stress-mediated p38 activation promotes somatic cell reprogramming." in: Cell research, Vol. 23, Issue 1, pp. 131-41, (2013) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Habib, Fares, Bourgeois, Bella, Bernardino, Hernandez-Blazquez, de Capoa, Niveleau: "DNA global hypomethylation in EBV-transformed interphase nuclei." in: Experimental cell research, Vol. 249, Issue 1, pp. 46-53, (1999) (PubMed).

Target Details

Target: 5-Methylcytosine

Target Type: Chemical