ATP6V0C antibody (Middle Region)

Catalog No. ABIN2773857

Product Details anti-ATP6V0C Antibody

Target: ATP6V0C (ATPase, H+ Transporting, Lysosomal 16kDa, V0 Subunit C (ATP6V0C))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Dog, Horse, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0C antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VVAVLIANSL NDDISLYKSF LQLGAGLSVG LSGLAAGFAI GIVGDAGVRG

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 93%, Human: 100%, Pig: 79%, Rabbit: 86%, Rat: 90%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0C. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6V0C

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 155 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6V0C

Target: ATP6V0C (ATPase, H+ Transporting, Lysosomal 16kDa, V0 Subunit C (ATP6V0C))

Alternative Name: ATP6V0C (ATP6V0C Products)

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the V0 domain. This gene had the previous symbols of ATP6C and ATP6L.
Alias Symbols: ATP6C, ATP6L, ATPL, VATL, Vma3, VPPC
Protein Interaction Partner: SMIM3, UBC, PSMA3, MSR1, EDA, CERS2, CLIC1, RNF182, MARK3, ARF6,
Protein Size: 155

Molecular Weight: 16 kDa

Gene ID: 527

NCBI Accession: NM_001694, NP_001685

UniProt: P27449

Pathways: Transition Metal Ion Homeostasis, Proton Transport