TAF6 antibody (N-Term)

Catalog No. ABIN2775955

Product Details anti-TAF6 Antibody

Target: TAF6 (TAF6 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 80kDa (TAF6))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Cow, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LTDEVSYRIK EIAQDALKFM HMGKRQKLTT SDIDYALKLK NVEPLYGFHA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against TAF6. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TAF6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 667 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TAF6 antibody (ABIN2775955)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for TAF6

Target: TAF6 (TAF6 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 80kDa (TAF6))

Alternative Name: TAF6 (TAF6 Products)

Background: TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF6 is one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1,the largest subunit of TFIID. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Four isoforms have been identified but complete transcripts have been determined for only three isoforms. One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis.
Alias Symbols: DKFZp781E21155, MGC:8964, TAF2E, TAFII70, TAFII80, TAFII85, TAFII-70, TAFII-80, TAF(II)70, TAF(II)80
Protein Interaction Partner: SOX5, HNF4A, MED26, TRRAP, HIST3H3, UBC, TBP, AHR, APP, TAF1L, TAF3, TAF9B, TAF10, TAF9, TAF7, TAF5, TAF4, TAF2, TAF1, KMT2A, USF1, ERCC1, TAF8, KAT2A, SETD7, NR1D2, NCOR1, NFYC, NFYB, NFYA, MBD3, SIN3A, ALL1, SAP30, SMARCC2, SMARCC1, SMARCB1, SMARCA2, RB
Protein Size: 667

Molecular Weight: 71 kDa

Gene ID: 6878

NCBI Accession: NM_139122, NP_620834

UniProt: A4D2B3