NR4A1 antibody (N-Term)

Catalog No. ABIN2777511

Product Details anti-NR4A1 Antibody

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GTPAPSPGPR DHLASDPLTP EFIKPTMDLA SPEAAPAAPT ALPSFSTFMD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NR4A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NR4A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 598 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR4A1 antibody (ABIN2777511)

Bian, Chen, Yang, Li, Zhang, Zhang, Wang, Zhao, Zhang, Chen, Zheng, Sun, Wang, Chien, Wu: "Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation." in: Nature communications, Vol. 8, pp. 14420, (2017) (PubMed).

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: NR4A1 (NR4A1 Products)

Background: NR4A1 encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.
Alias Symbols: HMR, N10, TR3, NP10, GFRP1, NAK-1, NGFIB, NUR77
Protein Interaction Partner: KRTAP10-5, KRTAP10-1, LONRF1, PGBD1, BCL2L10, CDKN2D, BCL2A1, BCL2, TUBB4A, VASP, ANKIB1, CUTA, NFKB1, SMAD3, LSS, HADHA, GLUD1, GK, CPT1A, SERPING1, ABCC6, ABCC13, SOCS4, ABCG8, DIABLO, HCVgp1, ABCA5, CHEK2, SUGT1, PRDX4, PRDX6, CHD1L, ABCB11, ARHGEF5, U
Protein Size: 598

Molecular Weight: 66 kDa

Gene ID: 3164

NCBI Accession: NM_173157, NP_775180

UniProt: P22736

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway