NR1D2 antibody (C-Term)

Catalog No. ABIN2780650

Product Details anti-NR1D2 Antibody

Target: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR1D2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ETLIRALRTL IMKNHPNEAS IFTKLLLKLP DLRSLNNMHS EELLAFKVHP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NR1D2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NR1D2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 579 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NR1D2

Target: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Alternative Name: NR1D2 (NR1D2 Products)

Background: NR1D2 can interact with NCOA5 coactivator, leading to a strong increase of transcription of target genes. NR1D2 also binds to the sequences 5'-AATGTAGGTCA-3' and 5'-ATAACTAGGTCA-3' and acts as a potent competitive transcriptional silencer and negative regulator of RORalpha mediated trans-activation. NR1D2 may play different roles in metabolism, inflammation, and circadian cycling in the organ-specific manner in homeostasis.
Alias Symbols: RVR, BD73, EAR-1R
Protein Interaction Partner: MID2, RBPMS, TRIM27, NOTCH2NL, KRTAP10-9, KRT40, KRTAP4-2, PRMT6, SKP1, UBC, SIN3B, KAT5, NCOR1, TAF9, TAF6, HDAC1, GTF2B, NCOA5, UBE2I, CUL1, NR1D1, RGN,
Protein Size: 579

Molecular Weight: 65 kDa

Gene ID: 9975

NCBI Accession: NM_005126, NP_005117

UniProt: Q14995

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway