YWHAZ antibody (Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (Middle Region)

Details for Product anti-YWHAZ Antibody No. ABIN2783207
Target
Binding Specificity
Middle Region
27
11
10
10
10
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish (Danio rerio)
145
106
84
20
18
16
16
10
6
6
5
5
3
3
2
2
1
1
Host
Rabbit
137
11
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
9
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
123
74
63
38
34
16
14
13
7
5
3
3
3
Options
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human YWHAZ
Blocking Peptide Blocking peptide for this product available: ABIN986309
Sequence FDEAIAELDT LSEESYKDST LIMQLLRDNL TLWTSDTQGD EAEAGEGGEN
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against YWHAZ. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Target
Alternative Name YWHAZ (YWHAZ Antibody Abstract)
Background YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
Alias Symbols: KCIP-1, MGC111427, MGC126532, MGC138156, YWHAD, 14-3-3-zeta
Protein Interaction Partner: FUS, UBC, HUWE1, MAPT, DTL, MAPK8, SGK1, ABL1, TSC2, DDIT4, SIK3, SIK2, SUMO2, STAU1, IRS4, MDM2, WWOX, YWHAZ, SNRPF, SNRPE, SLC9A3R1, LDHB, SFN, PFAS, PAK2, ERP44, MAPRE1, FERMT2, YWHAQ, PRMT5, YWHAH, YWHAG, VCL, UBE2V1, TGM2, EIF5, ECHS1, CAPNS1, ZFYVE1
Protein Size: 245
Molecular Weight 28 kDa
Gene ID 7534
NCBI Accession NM_003406, NP_003397
UniProt P63104
Pathways Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 245 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Images
Image no. 1 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (Middle Region) antibody (ABIN2783207) WB Suggested Anti-YWHAZ Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Image no. 2 for anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (Middle Region) antibody (ABIN2783207) anti-Tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (Middle Region) antibody (Image 2)