BRF2 antibody (N-Term)

Catalog No. ABIN2784260

Product Details anti-BRF2 Antibody

Target: BRF2 (BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRF2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VTEGVLTTTF SDEGNLREVT YSRSTGENEQ VSRSQQRGLR RVRDLCRVLQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BRF2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BRF2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 419 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BRF2 antibody (ABIN2784260)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for BRF2

Target: BRF2 (BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2))

Alternative Name: BRF2 (BRF2 Products)

Background: BRF2 is the general activator of RNA polymerase III transcription. BRF2 is the factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.
Alias Symbols: BRFU, FLJ11052, TFIIIB50
Protein Interaction Partner: UBC, SNAPC4, TBP, RBL2, IPO4, RNF219, XPO4, SMAP1, SMPD4, FANCI, ATAD3A, TMEM33, DNAAF5, OTUD4, PTPLAD1, GEMIN4, NOC2L, TNPO3, SF3B1, XPO6, XPO7, COPG1, GCN1L1, NUP93, AIFM1, DPM1, SLC3A2, ABCD3, MYO1B, MARS,
Protein Size: 419

Molecular Weight: 46 kDa

Gene ID: 55290

NCBI Accession: NM_018310, NP_060780

UniProt: Q9HAW0