BRF2 antibody (C-Term)

Catalog No. ABIN184848

The Goat Polyclonal anti-BRF2 antibody (ABIN184848) specifically detects BRF2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for BRF2 antibody (C-Term) (ABIN184848)

Target: BRF2 (BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BRF2 Antibody

Binding Specificity: C-Term

Purpose: BRF2

Sequence: QAARQAATSV PNPP

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QAARQAATSVPNPP, from the C Terminus of the protein sequence according to NP_060780.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in HeLa lysates (predicted MW of 48 kDa according to NP_060780). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BRF2

Target: BRF2 (BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2))

Alternative Name: BRF2

Background: BRF2, BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, BRFU, FLJ11052, TFIIIB50, transcription factor IIB- related factor, TFIIIB50, RNA polymerase III transcription initiation factor BRFU, RNA polymerase III transcription i

Gene ID: 55290

NCBI Accession: NP_060780