SOX9 antibody (N-Term)

Catalog No. ABIN2792587

Product Details anti-SOX9 Antibody

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PCPSGSGSDT ENTRPQENTF PKGEPDLKKE SEEDKFPVCI REAVSQVLKG

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against SOX9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SOX9

Application Details

Application Notes: WB Suggested Anti-SOX9 Antibody Titration: 2.5 µg/mL
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9 (SOX9 Products)

Background: SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Alias Symbols: CMD1, SRA1, CMPD1
Protein Interaction Partner: ZBTB7A, SCX, HERC1, UBC, SOX9, ATP6V1H, ctnnb1-a, RUNX2, SMAD3, SMAD2, EP300, CREB3L4, SPEN, KPNB1, TRAF2, CREBBP, MED12, NR5A1, MAF, HSPA1A, CREB1,
Protein Size: 509

Molecular Weight: 56 kDa

Gene ID: 6662

NCBI Accession: NM_000346, NP_000337

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development