SOX9 antibody (C-Term)

Catalog No. ABIN2780338

There is 1 publication for this product available. The Rabbit Polyclonal anti-SOX9 antibody is suitable to detect SOX9 in samples from Human, Mouse, Rat, Dog, Pig, Rabbit, Cow, Guinea Pig, Zebrafish (Danio rerio) and Horse. It has been validated for WB and FACS.

Quick Overview for SOX9 antibody (C-Term) (ABIN2780338)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit, Cow, Guinea Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SOX9 Antibody

Binding Specificity: C-Term

Purpose: SOX9 Antibody - C-terminal region

Sequence: AGQGTGLYST FTYMNPAQRP MYTPIADTSG VPSIPQTHSP QHWEQPVYTQ

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SOX9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SOX9

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8112844

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SOX9 antibody (ABIN2780338)

Lau, Lee, Chang: "Conserved sex-specific timing of meiotic initiation during sex differentiation in the protandrous black porgy Acanthopagrus schlegelii." in: Biology of reproduction, Vol. 88, Issue 6, pp. 150, (2013) (PubMed).

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background:

Background Information: SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: SRY (sex determining region Y)-box 9

Alternative Symbols: CMD1, SRA1, CMPD1, SRXX2, SRXY10

Protein Name: Transcription factor SOX-9

Molecular Weight: 56kDa

Gene ID: 6662

NCBI Accession: NP_000337

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development