COX4I1 antibody (Cytochrome C Oxidase Subunit IV Isoform 1) (Center)

Details for Product anti-COX4I1 Antibody No. ABIN2855069
Antigen
Epitope
Center
25
18
16
15
11
10
8
7
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
240
119
113
8
3
2
2
2
2
1
1
1
Host
Rabbit
185
54
7
2
Clonality
Polyclonal
Conjugate
This COX4I1 antibody is un-conjugated
11
9
8
5
5
5
5
5
5
5
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
194
99
79
58
43
42
28
15
13
13
12
3
3
2
1
Options
Immunogen Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Isotype IgG
Cross-Reactivity Chimpanzee
Cross-Reactivity (Details) Chimpanzee (100 %)
Characteristics Rabbit Polyclonal antibody to COX4 (cytochrome c oxidase subunit IV isoform 1)
COX4 antibody
Purification Purified by antigen-affinity chromatography.
Antigen
Alternative Name COX4 (COX4I1 Antibody Abstract)
Background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.

Cellular Localization: Mitochondrion inner membrane
Molecular Weight 20 kDa
Gene ID 1327
Pathways Proton Transport
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions For Research Use only
Format Liquid
Concentration 0.38 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (Center) antibody (ABIN2855069) IHC-P Image Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using ...
Image no. 2 for anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (Center) antibody (ABIN2855069) IP Image Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg...
Image no. 3 for anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (Center) antibody (ABIN2855069) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed H...
Image no. 4 for anti-Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) (Center) antibody (ABIN2855069) WB Image Sample(30 μg of whole cell lysate) A:A431, B:MOLT4, 12% SDS PAGE antibody di...