CYP17A1 antibody (Center)

Catalog No. ABIN2855475

Product Details anti-CYP17A1 Antibody

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP17A1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Chimpanzee

Cross-Reactivity (Details): Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to CYP17A1 (cytochrome P450, family 17, subfamily A, polypeptide 1)
CYP17A1 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CYP17A1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:20000*

Comment:

Positive Control: NIH-3T3 , C2C12 , MCF-7 , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CYP17A1

Target: CYP17A1 (Cytochrome P450, Family 17, Subfamily A, Polypeptide 1 (CYP17A1))

Alternative Name: CYP17A1 (CYP17A1 Products)

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia.

Cellular Localization: Membrane , Membrane, Single-pass membrane protein , Membrane , Membrane

Molecular Weight: 57 kDa

Gene ID: 1586

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin