UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 3 (B3GNT3) (Center) antibody

Details for Product No. ABIN2856643
Target
Binding Specificity
Center
4
3
2
1
Reactivity
Human, Mouse
20
9
Host
Rabbit
15
5
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
13
6
4
1
1
1
1
Options
Immunogen Recombinant protein encompassing a sequence within the center region of human B3GNT3. The exact sequence is proprietary.
Isotype IgG
Characteristics Rabbit Polyclonal antibody to B3GNT3 (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3)
B3GNT3 antibody
Purification Purified by antigen-affinity chromatography.
Target
Alternative Name B3GNT3 (B3GNT3 Antibody Abstract)
Background This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein and contains a signal anchor that is not cleaved. It prefers the substrates of lacto-N-tetraose and lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains and the biosynthesis of the backbone structure of dimeric sialyl Lewis a. It plays dominant roles in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking.

Cellular Localization: Golgi apparatus membrane
Molecular Weight 43 kDa
Gene ID 10331
Pathways Glycosaminoglycan Metabolic Process
Application Notes Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , mouse stomach

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Images
Image no. 1 for anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 3 (B3GNT3) (Center) antibody (ABIN2856643) IHC-P Image B3GNT3 antibody detects B3GNT3 protein at cytosol on human colon by immun...
Image no. 2 for anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 3 (B3GNT3) (Center) antibody (ABIN2856643) WB Image Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE antibody diluted at 1:1000
Image no. 3 for anti-UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 3 (B3GNT3) (Center) antibody (ABIN2856643) WB Image Sample (50 ug of whole cell lysate) A: mouse stomach 10% SDS PAGE antibody d...