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PAD4 antibody (Center)

Name: PAD4 antibody (Center)
Brand: antibodies-online
SKU: ABIN2856939
Price: 637.78 USD
Availability: InStock

PADI4 Reactivity: Human, Mouse WB, IF, ICC, IHC (p), ChIP Host: Rabbit Polyclonal unconjugated

WB Image PAD4 antibody detects PAD4 protein by western blot analysis. (PAD4 antibody (Center))

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4, antibody at 1:200 dilution. (PAD4 antibody (Center))

ChIP

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4, antibody at 1:100 dilution. (PAD4 antibody (Center))

IHC

IHC-P Image PAD4 antibody detects PAD4 protein at cytoplasm on mouse ovary by immunohistochemical analysis. (PAD4 antibody (Center))

IHC

5 images

(1 reference)

Catalog No. ABIN2856939

Datasheet as PDF

Target See all PAD4 (PADI4) Antibodies

PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))
Binding Specificity
15

5

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1
Center
Reactivity
61

9

8

2

1

1

1

1

1

1

1

1

1

1
Human, Mouse
Host
47

13

1
Rabbit
Compatible Secondaries
Clonality
51

11
Polyclonal
Conjugate
42

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PAD4 antibody is un-conjugated
Application
36

21

18

13

13

13

8

5

4

3

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Characteristics

Rabbit Polyclonal antibody to PAD4 (peptidyl arginine deiminase, type IV)
PAD4 antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAD4.
anti-Peptidyl Arginine Deiminase, Type IV (PADI4) antibody
PADI4 Reactivity: Human WB, IHC, IF, IHC (p), FACS Host: Mouse Monoclonal 4H5 unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody
Verified PADI4 Reactivity: Human IHC, ELISA, IF Host: Goat Polyclonal unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) antibody
PADI4 Reactivity: Human, Rat, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 374-550) antibody
PADI4 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 299-588) antibody
PADI4 Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5E7 unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 1-300) antibody
PADI4 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (N-Term) antibody
PADI4 Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 299-588) antibody
PADI4 Reactivity: Human WB, FACS Host: Mouse Monoclonal 5B7 unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 299-588) antibody
PADI4 Reactivity: Human WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3E1 unconjugated

anti-Peptidyl Arginine Deiminase, Type IV (PADI4) (AA 1-250) antibody
PADI4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000, ChIP.
Not yet tested in other applications.
Optimal working dilutions should be determined experimentally by the end user.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.22 mg/mL

Buffer

PBS

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.
Li, Lin, Deng, Strnad, Bernabei, Vogl, Burke, Nefedova: "A Novel Peptidylarginine Deiminase 4 (PAD4) Inhibitor BMS-P5 Blocks Formation of Neutrophil Extracellular Traps and Delays Progression of Multiple Myeloma." in: Molecular cancer therapeutics, Vol. 19, Issue 7, pp. 1530-1538, (2020) (PubMed).
Target

PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name

PAD4 (PADI4 Products)

Gene ID

23569