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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (Center) Primary Antibody

GAPDH Reactivity: Human, Mouse, Rat, Dog, Drosophila melanogaster, E. coli ICC, IF, IHC (rs), IP, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (104)
Catalog No. ABIN2857072
$509.08
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target
    GAPDH
    Binding Specificity
    • 41
    • 25
    • 16
    • 12
    • 11
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reactivity
    • 295
    • 184
    • 169
    • 78
    • 67
    • 63
    • 35
    • 34
    • 31
    • 27
    • 23
    • 20
    • 20
    • 19
    • 19
    • 15
    • 13
    • 10
    • 9
    • 8
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Drosophila melanogaster, E. coli
    Host
    • 191
    • 116
    • 24
    • 12
    • 6
    • 1
    Rabbit
    Clonality
    • 225
    • 124
    Polyclonal
    Conjugate
    • 197
    • 36
    • 24
    • 18
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GAPDH antibody is un-conjugated
    Application
    • 270
    • 134
    • 86
    • 73
    • 50
    • 47
    • 46
    • 37
    • 28
    • 28
    • 21
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Resin Sections) (IHC (rs)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Cross-Reactivity
    Cat (Feline), Chicken, Dog (Canine), Fruit Fly (Drosophila melanogaster), Monkey, Pig (Porcine), Rabbit, Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine)
    Cross-Reactivity (Details)
    Cat (96 %), Chicken (92 %), Dog (95 %), Monkey (99 %), Pig (95 %), Rabbit (94 %), Xenopus laevis (85 %), Zebrafish (86 %), Bovine (94 %)
    Characteristics
    Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
    GAPDH antibody
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:5000-1:100000* IHC (Resin sections) Assay-dependent dilution Immunoblotting Assay-dependent dilution Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:5000-1:100000* IHC (Resin sections)Assay-dependent dilution ImmunoblottingAssay-dependent dilution
    Comment

    Positive Control: E.coli M15 , 293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw 264.7 , C2Cl2 , PC-12 , Rat2 , *SH-SY5Y , *A549 , *U2OS , *H9C2 , *A7R5 , *mouse skin tissue

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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  • Target
    GAPDH
    Alternative Name
    GAPDH (GAPDH Antibody Abstract)
    Synonyms
    G3PD, GAPD, Gapd, BEST:GH12586, CG12055, Dmel\\CG12055, GA3PDH, GADPH, GAP, GAPDH, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, g3pd, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase S homeolog, glyceraldehyde-3-phosphate dehydrogenase, type I, olfactory receptor 8K3, GAPDH, Gapdh, Gapdh1, gapdh, gapdh.S, gapDH, LOC100404960, LOC614985
    Background
    The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

    Cellular Localization: Cytoplasm , perinuclear region , Membrane
    Molecular Weight
    36 kDa
    Gene ID
    2597
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