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GAPDH Antibodies (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Short Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).

Available GAPDH Antibodies:

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Center Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 GAPDH Reactivity: Human, Mouse, Rat, Dog, Drosophila melanogaster, E. coli ICC, IF, IHC (rs), IP, IHC (p), WB Host: Rabbit Polyclonal unconjugated

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in mouse testis by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH, antibody (green) at 1:500 dilution.

IP Image Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody, Western blot analysis was performed using GAPDH antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of human salivary gland cancer, using GAPDH, antibody at 1:500 dilution.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in rat heart by immunohistochemical analysis.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Effects of quercetin on the inflammatory response in H2O2-treated H9C2 cells.

Effects of quercetin on cell apoptosis in H2O2-treated H9C2 cells.

Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS.

Gene trap screen for mitochondrial phenotypes identifies Sucla2 mutant allele.

Sucla2 mutant embryos display growth deficiency and placental mtDNA depletion.

Sucla2-deficient embryo tissues exhibit progressive, functionally significant mtDNA depletion and elevated MMA.

Effects of quercetin on doxorubicin-induced changes of cell viability, cell apoptosis and cell morphology in H9C2 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

RAGE expression in human peripheral nerve fibers.

Arecoline induces the transdifferentiation of buccal mucosal fibroblasts (BMFs) into myofibroblasts.

Arecoline-induced α-smooth muscle actin (α-SMA) expression is mediated by the direct binding of zinc finger E-box binding homeobox 1 (ZEB1) to the α-SMA promoter.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Regulation of NED by REST in LNCaP cells.

IL-6 induces NED in LNCaP cells and this is concomitant with increased autophagy.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

Modulation of curcumin in oxidative stress-regulating genes.

Arecoline decreased membrane expression of ZO-1 in confluent TM4 cells.

Arecoline activates ERK1/2 MAPKs and increases TNF-alpha production.

Activation of ERK1/2 MAPKs is important for TNF-alpha induction and ZO-1 protein redistribution by arecoline in TM4 cells.

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway.

Early and accelerated processing of caspase-8 with DZNep treatment in lymphoma cell lines.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

Effects of different proteins on post-treatment lipolysis markers.

Creation and characterization of Supt4h knockout mice.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

The expression of BH3-only protein, pro-apoptotic proteins and anti-apoptotic proteins in LNCaP or PC3 cells after treatment with paclitaxel.

The effect of ABT-199, ABT-263 or ABT-263 in combination with paclitaxel on LNCaP or PC3 cells.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

Diverse fuels promote TNKS autoPARsylation and turnover in INS-1 cells.

Promoter hypermethylation of sGCβ1 in MCF-7 and sGCα1 in MDA-MB-231 cells.

Over-expression of sGCα1 and sGCβ1 in MDA-MB-231 cells affected cGMP production, proliferation, survival, and cell cycle.

Over-expression of sGC induced apoptosis and cell cycle arrest.

GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes.

Caspase-dependent apoptotic pathways were seen in LNCaP, but not in PC3 cells, in response to G2/M arrest.

LY294002 inhibits the HYS-32-induced phosphorylation of GSK3β-pS9 and GSK3β-pY216.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Effects of TLCA on HDAC4 expression in cultured primary HSCs.

Overexpression of miR-29a decreased HDAC4 expression in primary HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Western blot analysis shows pErk reduction through selumetinib treatment and by removal of stromal support.

Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Mek pathway inhibition in combination-treated BCP-ALL cells by phospho-flow and Western blot.

Demyelination/remyelination and decreased P0 expression in dKO mice.

Loss of neurofascins and Caspr in dKO mice.

Inhibition of ERK1/2 inhibits ETO-induced centrosome amplification.

Chloroquine inhibits DNA damage-induced centrosome amplification.

Chloroquine inhibits ETO-activated CDK2 and ERK1/2 in adrenocortical Y1 tumor cells.

HSPs positively regulate IE2 transaction activity.

Comparison of the mRNA levels of Smad7 (a) and protein levels of Smad3 (b) expressions in the liver of WT and miR-29Tg mice following bile duct ligation (BDL).

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

SFN suppressed HCV protein expression and RNA replication.

SFN-mediated HO-1 induction in HCV replicon cells.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN induced bilirubin production in HCV replicon cells.

SFN inhibited HCV replication by upregulating Nrf2 expression.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days.

Inhibition of neurite elongation by REST overexpression(A) LNCaP-TR-REST cells were treated with 0.

AMPK/mTOR pathway is activated by hypoxia treatment and REST knockdown(A) LNCaP cells were treated with normoxia (N) or hypoxia (H) (2 % O2) for 3 days (left panel).

Hypoxia-induced autophagy activation of LNCaP cells(A) LNCaP-eGFP-LC3 cells were treated with hypoxia (2 % O2) for 3 days.

Chemical inhibition of autophagy flux by chloroquine (CQ) suppresses hypoxia-induced NED in LNCaP cells(A) LNCaP cells were treated with hypoxia (2 % O2) in the absence or presence of 50 μM CQ for 3 days.

Knockdown of beclin 1 suppresses hypoxia-induced NED(A) LNCaP-TR-shBeclin1 cells were treated with 1 μg/mL Dox to induce beclin 1 knockdown for 4 days under normoxia and hypoxia (2 % O2).

Disruption of autophagy by treatment with CiQ derivativesA.

Induction of apoptosis by treatment with CiQ derivativesA.

Induction of lysosome dysfunction by treatment with CiQ derivativesA and B.

Differential expression of CDK1 pTyr15 in colorectal cancer cells and tissues.

Senataxin is SUMOylated at the XY chromosomes in spermatocytes.

120 images

105 references

Catalog No. ABIN2857072

$509.08

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100 μL

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 GAPDH Reactivity: Human, Mouse, Rat IF, WB Host: Mouse Monoclonal unconjugated

Slug is regulated by the co-transcriptional complex YAP1/TEAD in the EMT program of A549 cells.

Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:2000 dilution.

Western blot analysis of extracts of various cell lines, using GAPDH antibody.

Compared with CS, EGFL7 is highly expressed in OS.

YAP1 expression levels in NSCLC tissues and NSCLC cell lines.

EMT-related marker expression in A549 cells with YAP1 overexpression or silencing.

TEAD is involved in YAP1-induced EMT in A549 cells.

Inhibiting the co-transcription complex YAP/TEAD reverses A549 cell proliferation, migration, and invasion induced by TGF-β1.

The expressions of H19 and integrin β3 (ITGB3) decrease in human embryonic chorion tissue of spontaneous abortion.

Enhanced mitochondrial fission in STs of RA patients correlates with disease severity.

DNM1L deficiency alters mitochondria morphology and mitochondrial membrane potential in FLSs.

DNM1L deficiency in FLSs reduces their viability and production of pro-inflammatory cytokines, and increases apoptosis.

DNM1L deficiency reduces ROS production and autophagy in FLS.

DNM1L deficiency attenuates the IL-1β-induced NF-κB activation in FLSs.

HMGB1 was a direct target of miR-339-5p.

The effects of miR-339-5p and HMGB1-siRNA on LPS-induced RAW264.

Effects of paeonol on IKK-β in LPS-induced RAW264.

ISG20 promoted ccRCC cell proliferation in vitro.

ZNF139 and its circRNA (circZNF139) activates PI3K/AKT signaling pathway in BC cells.

Astrocytes were activated and mainly expressed as the A1 phenotype in the spinal cord after SMIR.

Minocycline reverted the A1/A2 ratio of reactive astrocytes and relieved mechanical allodynia in rats after SMIR.

The CXCR7 and PI3K/Akt signaling pathways are involved in CPSP in the dorsal horn of the spinal cord.

The combination of BKM120 and TH588 synergistically induces oxidative DNA damage and apoptosis.

Establishment and confirmation of radiation-induced lung fibrosis in a murine model.

Increased DIO2 expression in lung tissue after radiation treatment and inhibition of the TGF-β1 signaling pathway by thyroid hormone.

37 images

46 references

Catalog No. ABIN3020541

$435.60

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100 μL (More quantities available)

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) C-Term Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 Verified GAPDH Reactivity: Human, Rat ELISA, IF, IHC, WB Host: Goat Polyclonal unconjugated

ABIN185240 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN185240 (5µg/ml) staining of paraffin embedded Human Kidney.

ABIN185240 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

ABIN185240 (1µg/ml) staining of paraffin embedded Human Tonsil.

ABIN185240 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.

24 images

10 references

Catalog No. ABIN185240

$451.79

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100 μg

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) AA 136-335 Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 GAPDH Reactivity: Human, Mouse, Rat IHC (p), WB Host: Rabbit Polyclonal unconjugated

GAPDH was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL.

GAPDH was detected in paraffin-embedded sections of human thyroid cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL.

Western blot analysis of GAPDH expression in rat brain extract ( Lane 1), rat kidney extract ( Lane 2), mouse kidney extract ( Lane 3), HELA whole cell lysates ( Lane 4) and MCF-7 whole cell lysates( Lane 5).

3 images

115 references

Catalog No. ABIN5518757

$315.00

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100 μg

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 GAPDH Reactivity: Human, Mouse, Rat IF, IHC (p), WB Host: Mouse Monoclonal 1A10A10 unconjugated

Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35ug/lane).

Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(ABIN2838005).

Immunohistochemical analysis of paraffin-embedded H.

All lanes : Anti-GAPDH Antibody at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 μg per lane.

All lanes : Anti-GAPDH Antibody at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20 μg per lane.

6 images

12 references

Catalog No. ABIN1539789

$445.50

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400 μL (More quantities available)

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) Internal Region Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 Verified GAPDH Reactivity: Human, Mouse, Rat ELISA, IF, IHC, WB Host: Goat Polyclonal unconjugated

ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver.

ABIN185543 (2µg/ml) staining of paraffin embedded Human Liver.

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

11 images

6 references

Catalog No. ABIN185543

$451.79

Plus shipping costs $45.00

100 μg

Shipping to: United States

Delivery in 6 to 7 Business Days

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anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) N-Term Primary Antibody

Synonym: Glyceraldehyde 3 phosphate dehydrogenase 1 GAPDH Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep IHC, WB Host: Rabbit Polyclonal unconjugated