Heat Shock Protein 65 (HSP65) antibody

Details for Product No. ABIN2859571
Antigen
Reactivity
Mycobacteria
1
Host
Mouse
1
Clonality (Clone)
Monoclonal ()
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
1
1
Options
Immunogen Purified protein derivative (PPD)
Clone BDI577
Isotype IgG2a
Specificity This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
Characteristics Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
Purification Protein A Chromatography
Antigen
Alternative Name Heat Shock Protein 65 / HSP65 (HSP65 Antibody Abstract)
Background Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65
Gene ID 886354
UniProt P0A520
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL (OD280nm, E0.1% = 1.3)
Buffer 0.01 M PBS, pH 7.2 containing 0.09 % Sodium Azide without stabilizing proteins.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months