Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Heat Shock Protein 65 (HSP65) antibody Primary Antibody

HSP65 Reactivity: Mycobacteria EIA, WB Host: Mouse Monoclonal BDI578 unconjugated
Catalog No. ABIN2859572
$1,814.40
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 11 to 13 Business Days
  • Target
    Heat Shock Protein 65 (HSP65)
    Reactivity
    Mycobacteria
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Enzyme Immunoassay (EIA), Western Blotting (WB)
    Specificity
    This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
    Characteristics
    Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
    Purification
    Protein A chromatography
    Purity
    > 90 % pure
    Immunogen
    Purified protein derivative (PPD)
    Clone
    BDI578
    Isotype
    IgG2a
  • Application Notes
    ELISA. Western Blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0,1 mg/mL (OD280nm, E0.1% = 1.4)
    Buffer
    0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store the antibody at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Heat Shock Protein 65 (HSP65)
    Alternative Name
    Heat Shock Protein 65 / HSP65 (HSP65 Antibody Abstract)
    Synonyms
    CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, heat shock protein family D (Hsp60) member 1, HSPD1
    Background
    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65
    Gene ID
    886354
    UniProt
    P0A520
    Pathways
    Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
You are here:
help Support