Integrin-Binding Sialoprotein (IBSP) (C-Term) antibody

Details for Product No. ABIN3031384
Target
Binding Specificity
C-Term
15
14
9
7
4
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Mouse, Rat
79
25
22
2
2
Host
Rabbit
70
4
2
2
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
34
18
13
12
10
8
8
8
2
2
2
Options
Immunogen An amino acid sequence from the C-terminus of mouse Bone Sialoprotein 2 (SYYKGHGYEGYEGQNYYYHQ) was used as the immunogen for this IBSP antibody.
Isotype IgG
Purification Antigen affinity
Target
Alternative Name IBSP / Bone Sialoprotein2 (IBSP Antibody Abstract)
Background Integrin-binding sialoprotein, also known as Bone sialoprotein and BSP, is a major structural protein of the bone matrix. Bone sialoprotein is a predicted 35KD acidic glycoprotein that undergoes extensive posttranslational modifications and is visualized by western blot at up to 70KD. It constitutes approximately 12 % of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
UniProt Q61711
Application Notes The stated application concentrations are suggested starting amounts. Titration of the IBSP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions For Research Use only
Buffer 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage -20 °C
Storage Comment After reconstitution, the IBSP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Images
Image no. 1 for anti-Integrin-Binding Sialoprotein (IBSP) (C-Term) antibody (ABIN3031384) Western blot testing of IBSP antibody and Lane 1: rat liver