IBSP antibody (Native Form)

Catalog No. ABIN191977

The Mouse Monoclonal anti-IBSP antibody (Clone ID1-2) (ABIN191977) specifically detects IBSP in WB, IHC (p), EIA, IHC (fro), IF and RIA. The antibody is reactive with Human samples.

Quick Overview for IBSP antibody (Native Form) (ABIN191977)

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IBSP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Clone: ID1-2

Product Details anti-IBSP Antibody

Binding Specificity: Native Form

Specificity: This antibody recognizes Human Bone Sialoprotein, Recombinant Human, Recombinant Porcine and Recombinant Rat BSP. There was no cross reactivity obtained with Human, Porcine Osteonectin and Osteopontin.

Cross-Reactivity (Details): Species reactivity (tested):Human, Porcine, Rat.

Characteristics: Synonyms: Bone sialoprotein II, BSP II, IBSP, BNSP, Cell-binding sialoprotein, Integrin-bindingsialoprotein

Purification: Protein G Chromatography

Immunogen: Human Bone Sialoprotein (Bone extract).

Isotype: IgG1

Application Details

Application Notes: ELISA (3). RIA. Western blot: 1/1000 (1,3). Immunocytochemstry: 1/200 (3). Immunohistochemistry on CryoSections, Resin-Embedded Sections: 1/100 (2,3,5,6). Immunohistochemistry on Paraffin Sections: 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in aqua bidest to 1 mg/mL.

Buffer: 50 mM TRIS, pH 7.4

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for IBSP

Target: IBSP (Integrin-Binding Sialoprotein (IBSP))

Alternative Name: Bone Sialoprotein 2

Background: The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12 % of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.Synonyms: BNSP, BSP II, Bone sialoprotein II, Cell-binding sialoprotein, IBSP, Integrin-binding sialoprotein

Gene ID: 3381

UniProt: P21815