p65 antibody (Nuclear Factor-KB P65) (AA 99-551)

Details for Product anti-NFkBP65 Antibody No. ABIN3042741
Antigen
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Alternatives
anti-Human p65 antibody for Immunoprecipitation
Epitope
AA 99-551
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80
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Reactivity
Human
1210
747
606
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Host
Rabbit
1140
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11
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Clonality
Polyclonal
Conjugate
This p65 antibody is un-conjugated
45
28
26
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20
13
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11
8
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3
2
2
Application
Western Blotting (WB)
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Options
Purpose Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Human.
Immunogen E.coli-derived human NF-kB p65 recombinant protein (Position: F99-S551). Human NF-kB p65 shares 86% amino acid (aa) sequence identity with mouse NF-kB p65.
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB in Human.
Gene Name: v-rel avian reticuloendotheliosis viral oncogene homolog A
Protein Name: Transcription factor p65
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others p65 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name RELA (NFkBP65 Antibody Abstract)
Background Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein that in humans is encoded by the RELA gene. It is mapped to 11q13.1. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

Synonyms: Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody|MGC131774 antibody|NF kappa B p65delta3 antibody|NFKB 3 antibody|NFKB3 antibody|Nuclear Factor NF Kappa B p65 Subunit antibody|Nuclear factor NF-kappa-B p65 subunit antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody|Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody|OTTHUMP00000233473 antibody|OTTHUMP00000233474 antibody|OTTHUMP00000233475 antibody|OTTHUMP00000233476 antibody|OTTHUMP00000233900 antibody|p65 antibody|p65 NF kappaB antibody|p65 NFkB antibody|RELA antibody|TF65_HUMAN antibody|Transcription Factor p65 antibody|v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody|V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody|v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody|V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody|v-rel reticuloendotheliosis viral oncogene homolog A antibody
Gene ID 4790
UniProt Q04206
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for NF-kB p65 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 99-551) antibody (ABIN3042741) Observed bind size: 64KD
Product cited in: "Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, 2018 (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "[Corrigendum] Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6926, 2018 (PubMed).

Wang, Liu, Wang, Wang, Tang, Jiang: "Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1282-1294, 2018 (PubMed).

Liu, Bao, Zeng, Wei: "Effects of resveratrol and genistein on nuclear factor‑κB, tumor necrosis factor‑α and matrix metalloproteinase‑9 in patients with chronic obstructive pulmonary disease." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4266-72, 2017 (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, 2017 (PubMed).

Wu, Gao, Li, Yao, Wang, Chen, Li, Ma, Ji, Li: "Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2715718, 2017 (PubMed).

Xie, Zhong, Li: "Comment on "Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats"." in: Journal of diabetes research, Vol. 2016, pp. 7302620, 2017 (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, 2017 (PubMed).

Yang, Gao, Wu, Yu, Li, Meng, Li, Yan, Jin: "Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 1975-1982, 2017 (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, 2017 (PubMed).

Chen, Liu, Wang, Feng, Zhao, Shi, Liu: "Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury." in: Oxidative medicine and cellular longevity, Vol. 2015, pp. 106836, 2016 (PubMed).

Huang, Zhong, Zhang, Wang, Xu, Zhu, Wang, Hu, Zhao: "Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-κB pathway." in: Scientific reports, Vol. 5, pp. 12807, 2016 (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, 2016 (PubMed).

Zuo, Zheng, Liu, Ma: "Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10653-61, 2016 (PubMed).

Wendan, Changzhu, Xuhong, Hongjing, Hong, Dongxia, Fang: "BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation." in: Stem cells international, Vol. 2016, pp. 3196071, 2016 (PubMed).

Wang, Li, Zhen, Xu, Sun: "Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy." in: International journal of clinical and experimental pathology, Vol. 8, Issue 12, pp. 15696-707, 2016 (PubMed).

Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 8219287, 2016 (PubMed).

Ge, Cao, Wang, Sun, Sun, Han, Cui, Liu: "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation." in: OncoTargets and therapy, Vol. 9, pp. 1011-21, 2016 (PubMed).

Li, Zhang, Qi, Zhang, Yi, Li, Zhang: "Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB." in: The journal of headache and pain, Vol. 17, pp. 49, 2016 (PubMed).

Wang, Su, Che, Zheng, Shi: "Penehyclidine ameliorates acute lung injury by inhibiting Toll-like receptor 2/4 expression and nuclear factor-κB activation." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1827-1832, 2016 (PubMed).

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