EIF3B antibody (C-Term)

Catalog No. ABIN3042942

Product Details anti-EIF3B Antibody

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Binding Specificity: AA 755-775, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 3 subunit B(EIF3B) detection. Tested with WB in Human,Mouse,Rat.

Sequence: EDFRKYRKMA QELYMEQKNE R

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 3 subunit B(EIF3B) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: eukaryotic translation initiation factor 3, subunit B
Protein Name: Eukaryotic translation initiation factor 3 subunit B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human eIF3B(755-775aa EDFRKYRKMAQELYMEQKNER), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for EIF3B is approximately 2.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF3B

Target: EIF3B (Eukaryotic Translation Initiation Factor 3, Subunit B (EIF3B))

Alternative Name: EIF3B (EIF3B Products)

Background: EIF3B(Eukaryotic Translation Initiation Factor 3, Subunit B), also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al.(1997) identified a cDNA encoding a human Prt1 homolog. Asano et al.(1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of 2 proteins, p116(PRT1) and an unrelated 110-kD protein. Chaudhuri et al.(1997) isolated cDNAs encoding PRT1, which they called p110 based on the size of the corresponding component of rabbit eIF3.

Synonyms: eIF-3-eta antibody|eIF3 eta antibody|eIF3 p110 antibody|eIF3 p116 antibody|EIF3-ETA antibody|EIF3-P110 antibody|EIF3-P116 antibody|eIF3b antibody|EIF3B_HUMAN antibody|EIF3S9 antibody|Eukaryotic translation initiation factor 3, subunit B antibody|Eukaryotic translation initiation factor 3 subunit 9 antibody|eukaryotic translation initiation factor 3 subunit 9 eta antibody|Eukaryotic translation initiation factor 3 subunit B antibody|hPrt1 antibody|PRT1 antibody|Prt1 homolog antibody

UniProt: P55884

Pathways: Ribonucleoprotein Complex Subunit Organization