CD59 antibody (AA 26-102)

Catalog No. ABIN3043396

This Rabbit Polyclonal antibody specifically detects CD59 in WB, FACS, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CD59 antibody (AA 26-102) (ABIN3043396)

Target: CD59

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CD59 Antibody

Binding Specificity: AA 26-102

Purpose: Anti-CD59 glycoprotein CD59 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD59 glycoprotein CD59 Antibody (ABIN3043396). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD59 recombinant protein (Position: L26-N102). Human CD59 shares 47.1% amino acid (aa) sequence identity with rat CD59.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶cells
1. "Entrez Gene: CD59 Molecule, complement regulatory protein". 2. Ninomiya H, Sims PJ (1992). "The human complement regulatory protein CD59 binds to the alpha-chain of C8 and to the "b"domain of C9". J. Biol. Chem. 267 (19): 13675-80.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background:

Synonyms: CD59 glycoprotein,1F5 antigen,20 kDa homologous restriction factor,HRF-20,HRF20,MAC-inhibitory protein,MAC-IP,MEM43 antigen,Membrane attack complex inhibition factor,MACIF,Membrane inhibitor of reactive lysis,MIRL,Protectin,CD59,CD59,MIC11, MIN1, MIN2, MIN3, MSK21,

Background: This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Sequence Similarities: Contains 1 UPAR/Ly6 domain.

Molecular Weight: 19 kDa

Gene ID: 966

UniProt: P13987

Pathways: Complement System