CD59 antibody (CD59)

Details for Product anti-CD59 Antibody No. ABIN94203
Antigen
Reactivity
Human, Mouse (Murine)
459
27
25
10
8
7
3
2
2
1
1
Host
Mouse
332
156
15
8
Clonality (Clone)
Monoclonal ()
Conjugate
This CD59 antibody is un-conjugated
50
47
47
17
8
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
329
159
122
119
93
82
64
39
38
32
28
15
13
11
8
4
3
2
2
1
1
1
Options
Immunogen Thymocytes and T lymphocytes
Clone MEM-43-5
Isotype IgG2b
Specificity The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues.
The MEM-43/5 does not compete with most other CD59 antibodies.
HLDA V, WS Code AS S012
Cross-Reactivity (Details) Mouse, Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Pathways Complement System
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD59 (CD59) antibody (ABIN94203) Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL h...
Product cited in: Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997 (PubMed).

Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, 1997 (PubMed).