Angiopoietin 2 antibody (C-Term)

Catalog No. ABIN3043679

Product Details anti-Angiopoietin 2 Antibody

Target: Angiopoietin 2 (ANGPT2)

Binding Specificity: AA 454-468, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Angiopoietin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with WB in Human.

Sequence: NLNGMYYPQR QNTNK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Angiopoietin-2(ANGPT2) detection. Tested with WB in Human.
Gene Name: angiopoietin 2
Protein Name: Angiopoietin-2(ANG-2)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Angiopoietin 2(454-468aa NLNGMYYPQRQNTNK).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Angiopoietin 2 antibody (ABIN3043679)

Huang, Pan, Yu, Guo, Wang, Zhang, Wang, Gao: "Benefical therapeutic effect of Chinese Herbal Xinji'erkang formula on hypertension-induced renal injury in the 2-kidney-1-clip hypertensive rats." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 11, Issue 5, pp. 16-27, (2015) (PubMed).

Bai, Li, Tian, Zhou: "Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes." in: PLoS ONE, Vol. 9, Issue 4, pp. e96117, (2014) (PubMed).

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, (2013) (PubMed).

Target Details for Angiopoietin 2

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANGPT2 (ANGPT2 Products)

Synonyms: AGPT2 antibody, ANG2 antibody, Agpt2 antibody, Ang-2 antibody, Ang2 antibody, ang2 antibody, angpt2 antibody, ANGIOPOIETIN-2 antibody, ANG-2 antibody, ANGPT2 antibody, Angrp antibody, Rnase5b antibody, ANG3 antibody, C11orf2 antibody, C11orf3 antibody, FFR antibody, angiopoietin 2 antibody, angiopoietin 2b antibody, angiogenin, ribonuclease A family, member 2 antibody, VPS51, GARP complex subunit antibody, ANGPT2 antibody, Angpt2 antibody, angpt2b antibody, Ang2 antibody, VPS51 antibody

Background: Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60 % homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Synonyms: AGPT 2 antibody|Agpt2 antibody|ANG 2 antibody|ANG-2 antibody|ANG2 antibody|Angiopoietin 2a antibody|Angiopoietin 2B antibody|Angiopoietin-2 antibody|Angiopoietin2 antibody|ANGP2_HUMAN antibody|ANGPT 2 antibody|ANGPT2 antibody|ANGPT-2 antibody|Tie2 ligand antibody

UniProt: O15123

Pathways: RTK Signaling