Myelin Basic Protein (MBP) (AA 182-197), (C-Term) antibody

Details for Product No. ABIN3043688
Antigen
  • C76307
  • Hmbpr
  • R75289
  • golli-mbp
  • mld
  • shi
  • Mbps
  • cb274
  • fj33b11
  • mbp
  • wu:fj33b11
  • wu:fq15b02
  • zgc:136630
  • MGC115135
  • myelin basic protein
  • myelin basic protein a
  • myelin basic protein L homeolog
  • MBP
  • Mbp
  • mbpa
  • mbp
  • mbp.L
Epitope
AA 182-197, C-Term
32
17
15
15
14
10
9
9
8
7
6
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
282
112
96
50
25
20
16
15
10
8
6
6
6
5
3
2
2
2
1
1
Host
Rabbit
205
95
17
13
6
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
14
12
11
6
5
4
4
4
4
4
4
4
3
3
3
3
3
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
209
128
122
58
47
39
31
30
20
20
17
17
11
10
6
3
3
1
1
Options
Purpose Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.
Sequence KLGGRDSRSG SPMARR
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: myelin basic protein
Protein Name: Myelin basic protein(MBP)
Purification Immunogen affinity purified.
Antigen
Alternative Name Myelin basic protein (MBP Antibody Abstract)
Background Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Synonyms: GDB antibody|Golli MBP antibody|Hemopoietic MBP antibody|HMBPR antibody|HUGO antibody|MBP antibody|MBP_HUMAN antibody|MGC99675 antibody|MLD antibody|Myelin A1 Protein antibody|Myelin basic protein antibody|Myelin Deficient antibody|Myelin Membrane Encephalitogenic Protein antibody|OTTHUMP00000163776 antibody|OTTHUMP00000174387 antibody|OTTHUMP00000174388 antibody|SHI antibody|Shiverer antibody|SP antibody
UniProt P02686
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Image no. 1 for anti-Myelin Basic Protein (MBP) (AA 182-197), (C-Term) antibody (ABIN3043688) Anti-Myelin Basic Protein antibody, Western blotting WB: Mouse Brain Tissue Lysate
Image no. 2 for anti-Myelin Basic Protein (MBP) (AA 182-197), (C-Term) antibody (ABIN3043688) Anti-Myelin Basic Protein antibody, IHC(P) IHC(P): Rat Brain Tissue
Product cited in: Zhou, Liu, Wang, Zou, Wei, Zhou: "Neuroprotective effect of ischemic postconditioning on sciatic nerve transection." in: Neural regeneration research, Vol. 13, Issue 3, pp. 492-496, 2018 (PubMed).

Mi, Gao, Liu, Ye, Li, Jin, Yang, Yang: "Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 20, pp. 2780-91, 2017 (PubMed).

Hu, Zhang, Tian, Xun, Wang, Lv: "Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 49-58, 2016 (PubMed).

Jin, Liu, Deng, Liao, Lin, Ning, Luo et al.: "Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin ..." in: International journal of molecular medicine, Vol. 35, Issue 5, pp. 1199-212, 2015 (PubMed).

Chen, Chen, Huang, Qin, Fang, Liu, Zhang, Pan, Wang, Xie: "Soluble epoxide hydrolase inhibition provides multi-target therapeutic effects in rats after spinal cord injury." in: Molecular neurobiology, 2015 (PubMed).

Zhang, Chai, Liu, Xu, Yang: "Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1141-1148, 2015 (PubMed).

Yang, Gao, Zhang, Fang, Wu, Xu, Huang: "Changes in proinflammatory cytokines and white matter in chronically stressed rats." in: Neuropsychiatric disease and treatment, Vol. 11, pp. 597-607, 2015 (PubMed).

Gao, Zheng, Cai, Yao, Wang, Zhang, Wang: "Comparison of morphology and biocompatibility of acellular nerve scaffolds processed by different chemical methods." in: Journal of materials science. Materials in medicine, Vol. 25, Issue 5, pp. 1283-91, 2014 (PubMed).

Han, Wan, Kudo, Foltz, Vines, Green, Zoerle, Tariq, Brathwaite, DAbbondanza, Ai, Macdonald: "Molecular alterations in the hippocampus after experimental subarachnoid hemorrhage." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 34, Issue 1, pp. 108-17, 2014 (PubMed).

Wei, Zheng, Liang, Wei, Yin, Tang, Xue: "Apolipoprotein E and its mimetic peptide suppress Th1 and Th17 responses in experimental autoimmune encephalomyelitis." in: Neurobiology of disease, Vol. 56, pp. 59-65, 2013 (PubMed).

Wang, Tu, Zhao, An, Wang, Liu, Yuan, Ahmed, Xu, Liu, Zhang, Wang: "Effects of poly(L-lysine)-modified Fe3O4 nanoparticles on endogenous reactive oxygen species in cancer stem cells." in: Biomaterials, Vol. 34, Issue 4, pp. 1155-69, 2012 (PubMed).

Ping, Yao, Jiang, Zhao, Yu, Jiang, Lin, Chen, Wang, Zhang, Cui, Qian, Wang, Bian: "The chemokine CXCL12 and its receptor CXCR4 promote glioma stem cell-mediated VEGF production and tumour angiogenesis via PI3K/AKT signalling." in: The Journal of pathology, Vol. 224, Issue 3, pp. 344-54, 2011 (PubMed).

Wang, Xiao, Chen, Han, Gao, Zhang, Zhao, Wang, Dai: "Nogo-66 promotes the differentiation of neural progenitors into astroglial lineage cells through mTOR-STAT3 pathway." in: PLoS ONE, Vol. 3, Issue 3, pp. e1856, 2008 (PubMed).

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