MFGE8 antibody (Middle Region)

Catalog No. ABIN3044017

Product Details anti-MFGE8 Antibody

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Binding Specificity: AA 165-180, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.

Sequence: NGHEFDFIHD VNKKHK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.
Gene Name: milk fat globule-EGF factor 8 protein
Protein Name: Lactadherin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1(165-180aa NGHEFDFIHDVNKKHK).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MFGE8 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-MFGE8 antibody (ABIN3044017)

Becker-Dreps, Choi, Stamper, Vilchez, Velasquez, Moon, Hudgens, Jiang, Permar: "Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants." in: Journal of the Pediatric Infectious Diseases Society, Vol. 6, Issue 1, pp. 87-90, (2018) (PubMed).

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFGE8 (MFGE8 Products)

Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

Synonyms: BA 46 antibody|BA46 antibody|Breast epithelial antigen BA46 antibody|Contains Medin antibody|EDIL 1 antibody|EDIL1 antibody|HMFG antibody|hP47 antibody|HsT19888 antibody|Lactadherin antibody|Lactadherin precursor antibody|Medin antibody|MFG E8 antibody|MFG-E8 antibody|MFGE 8 antibody|MFGE8 antibody|MFGE8 protein antibody|MFGM antibody|MFGM_HUMAN antibody|Milk fat globule EGF factor 8 antibody|Milk fat globule EGF factor 8 protein antibody|Milk fat globule-EGF factor 8 antibody|O acetyl disialoganglioside synthase antibody|OAcGD3S antibody|SED1 antibody|SPAG10 antibody|Sperm associated antigen 10 antibody|Sperm surface protein hP47 antibody

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome