FAS antibody (N-Term)

Catalog No. ABIN3044338

Product Details anti-FAS Antibody

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Binding Specificity: AA 87-109, N-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.

Sequence: YTDRKHYSDK CRRCAFCDEG HGL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P, IHC-F in Mouse,Rat.
Gene Name: Fas(TNF receptor superfamily, member 6)
Protein Name: Tumor necrosis factor receptor superfamily member 6

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas(87-109aa, YTDRKHYSDKCRRCAFCDEGHGL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat , Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-FAS antibody (ABIN3044338)

Yeung, Wang, Yao, Liang, Tenny Chung, Chuai, Lee, Yang: "BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse." in: Cell death & disease, Vol. 8, Issue 3, pp. e2697, (2017) (PubMed).

Gao, Liu, Huang, Zhang, Mei, Wang, Gong, Zhao, Xie, Ma, Qian: "HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1." in: Cell stress & chaperones, Vol. 20, Issue 4, pp. 653-61, (2016) (PubMed).

Liu, Xu, Zhao, Xia, Song, Gu, Liu, Bian, Yuan, Liu: "Osteoprotegerin Induces Apoptosis of Osteoclasts and Osteoclast Precursor Cells via the Fas/Fas Ligand Pathway." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142519, (2016) (PubMed).

Li, Xu, Jiang, Zhao, Ge, Zheng: "Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 11915-20, (2016) (PubMed).

Li, Wu, Sun, Zhao, Liu, Zhang: "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells" in: Asian Pacific journal of cancer prevention : APJCP, Vol. 17, Issue 11, pp. 4893-4898, (2016) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Liu, Su, Zhang, Zhang, Wang, Qian: "Rhodiola rosea suppresses thymus T-lymphocyte apoptosis by downregulating tumor necrosis factor-?-induced protein 8-like-2 in septic rats." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 386-98, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Wu, Hu, Liu, Cao, Xu, Li, Li: "Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice." in: International journal of molecular medicine, Vol. 33, Issue 6, pp. 1498-506, (2014) (PubMed).

Weng, Xie, Yi, Huang, Luo, Du, Chen, Liu, Chen, Chen: "Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 22, Issue 8, pp. 1197-205, (2014) (PubMed).

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Ma, Zhang, Zhang, Yang, Meng, Zhang, Zhou: "The effect of esculentoside A on lupus nephritis-prone BXSB mice." in: Archives of medical science : AMS, Vol. 9, Issue 2, pp. 354-60, (2013) (PubMed).

Liu, Liu, Li, Wang, Zhao: "Hydrogen sulfide donor regulates alveolar epithelial cell apoptosis in rats with acute lung injury." in: Chinese medical journal, Vol. 126, Issue 3, pp. 494-9, (2013) (PubMed).

Li, Xia, Wang, Li, Xue, Wu, Ye: "Relationship between aberrant methylation of FAS promoter and biological behavior of bladder urothelial carcinoma." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 31, Issue 6, pp. 794-8, (2011) (PubMed).

Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, (2010) (PubMed).

Li, Chu, Gao, Li: "Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin." in: Acta biochimica et biophysica Sinica, Vol. 42, Issue 1, pp. 80-9, (2009) (PubMed).

Liu, Zhang, Zhang, Zhu, Tian: "Construction and identification of Fas-targeting siRNA-expressing plasmid." in: Journal of Zhejiang University. Science. B, Vol. 6, Issue 7, pp. 673-7, (2005) (PubMed).

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: FAS (FAS Products)

Background: FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Synonyms: ALPS 1A antibody|ALPS1A antibody|APO 1 antibody|Apo 1 antigen antibody|APO 1 cell surface antigen antibody|Apo-1 antigen antibody|APO1 antibody|Apo1 antigen antibody|APO1 cell surface antigen antibody|Apoptosis antigen 1 antibody|Apoptosis mediating surface antigen FAS antibody|Apoptosis-mediating surface antigen FAS antibody|APT 1 antibody|APT1 antibody|CD 95 antibody|CD 95 antigen antibody|CD95 antibody|CD95 antigen antibody|Delta Fas antibody|Delta Fas/APO 1/CD95 antibody|Delta Fas/APO1/CD95 antibody|Fas(TNF receptor superfamily, member 6) antibody|FAS 1 antibody|FAS 827dupA antibody|Fas AMA antibody|FAS antibody|FAS Antigen antibody|Fas cell surface death receptor antibody|FAS1 antibody|FASLG receptor antibody|FASTM antibody|sFAS antibody|Surface antigen APO1 antibody|TNF receptor superfamily, member 6 antibody|TNFRSF 6 antibody|TNFRSF6 antibody|TNR6_HUMAN antibody|Tumor necrosis factor receptor superfamily member 6 antibody

UniProt: Q63199

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity