SOD1 antibody (C-Term)

Catalog No. ABIN3044379

This anti-SOD1 antibody is a Rabbit Polyclonal antibody detecting SOD1 in WB, IHC and ICC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 6+ publications.

Quick Overview for SOD1 antibody (C-Term) (ABIN3044379)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SOD1 Antibody

Binding Specificity: AA 113-129, C-Term

Purpose: Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband®

Sequence: IIGRTLVVHE KADDLGK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Superoxide Dismutase 1/SOD1 Antibody (ABIN3044379). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Crapo, J. D., Oury, T., Rabouille, C., Slot, J. W., Chang, L.-Y. Copper,zinc superoxide dismutase is primarily a cytosolic protein in human cells. Proc. Nat. Acad. Sci. 89: 10405-10409, 1992.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SOD1 antibody (ABIN3044379)

Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." in: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Wu, Li, Cui, Wu, Hong, Li, Wu, Jie, Wang, Li: "The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway." in: Frontiers in pharmacology, Vol. 9, pp. 497, (2018) (PubMed).

Hou, Zheng, Li, Gao, Zhang: "The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15026-43, (2015) (PubMed).

Guo, Qi, Yu, Du, Wu, Liu: "Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats." in: Diabetology & metabolic syndrome, Vol. 6, Issue 1, pp. 29, (2014) (PubMed).

Long, Yu, Shuai, Guo, Duan, Xu, Li: "The hypoglycemic effect of the kelp on diabetes mellitus model induced by alloxan in rats." in: International journal of molecular sciences, Vol. 13, Issue 3, pp. 3354-65, (2012) (PubMed).

Chen, Chen, Qin: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background:

Synonyms: Superoxide dismutase [Cu-Zn],1.15.1.1,Superoxide dismutase 1,hSod1,SOD1,

Tissue Specificity: Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

Background: Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Sequence Similarities: Belongs to the Cu-Zn superoxide dismutase family.

Molecular Weight: 18 kDa

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis