GABRA1 antibody (C-Term)

Catalog No. ABIN3044445

Product Details anti-GABRA1 Antibody

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Binding Specificity: AA 440-456, C-Term

Reactivity: Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Gamma-aminobutyric acid receptor subunit alpha-1(GABRA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: ATYLNREPQL KAPTPHQ

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Gamma-aminobutyric acid receptor subunit alpha-1(GABRA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: gamma-aminobutyric acid(GABA) A receptor, alpha 1
Protein Name: Gamma-aminobutyric acid receptor subunit alpha-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1(440-456aa ATYLNREPQLKAPTPHQ), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-GABRA1 antibody (ABIN3044445)

Bao, Wang, Wei, Wang, Wuyun, Bo: "Role of microRNA-4458 in patients with non-small-cell lung cancer." in: Oncology letters, Vol. 12, Issue 5, pp. 3958-3966, (2016) (PubMed).

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABRA1 (GABRA1 Products)

Background: GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97 % homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.

Synonyms: ECA4 antibody|EJM antibody|EJM5 antibody|Gaba receptor alpha 1 polypeptide antibody|GABA(A) receptor antibody|GABA(A) receptor subunit alpha 1 antibody|GABA(A) receptor subunit alpha-1 antibody|GABA(A) receptor, alpha 1 antibody|GABRA 1 antibody|GABRA1 antibody|Gamma aminobutyric acid(GABA) A receptor alpha 1 antibody|Gamma aminobutyric acid A receptor alpha 1 antibody|Gamma aminobutyric acid receptor subunit alpha 1 antibody|Gamma-aminobutyric acid receptor subunit alpha-1 antibody|GBRA1_HUMAN antibody

UniProt: P14867