ABCB11 antibody (AA 705-718)

Catalog No. ABIN305775

Product Details anti-ABCB11 Antibody

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Binding Specificity: AA 705-718

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCB11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human BSEP. Does not cross-react with human P-glycoprotein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%) Marmoset, Dog (93%).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%), Marmoset, Dog (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS  pH 7.2, 0.7 % BSA, 100nM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABCB11

Target: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Alternative Name: ABCB11 / BSEP (ABCB11 Products)

Background: Name/Gene ID: ABCB11
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB11, ABC16, Bile salt export pump, BSEP, PGY4, Sister p-glycoprotein, BRIC2, PFIC-2, PFIC2, SPGP

Gene ID: 8647

UniProt: O95342