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BRF1 antibody (BRF1, RNA polymerase III transcription initiation factor subunit) Primary Antibody

BRF1 Reactivity: Human, Mouse IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN317615
$513.70
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    BRF1
    Reactivity
    • 43
    • 25
    • 16
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 58
    • 6
    Rabbit
    Clonality
    • 60
    • 4
    Polyclonal
    Conjugate
    • 33
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BRF1 antibody is un-conjugated
    Application
    • 45
    • 25
    • 13
    • 12
    • 12
    • 10
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody detects endogenous levels of TFIIIB90-1 protein.(region surrounding Glu257)
    Purification
    Affinity Chromatography using epitope-specific immunogen
  • Application Notes
    Western Blot: 1/500-1/1000. ELISA: 1/1000-1/5000. Immunohistochemistry: 1/50-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    BRF1
    Alternative Name
    BRF1 / TFIIIB90 (BRF1 Antibody Abstract)
    Synonyms
    BRF, BRF-1, GTF3B, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF, DDBDRAFT_0189159, DDBDRAFT_0216283, DDB_0189159, DDB_0216283, 2510002F24Rik, mTFIIIB90, BRF1, ZFP36L1, CG31256, CG4155, CG5419, Dmel\\CG31256, TFIIIB, brf, brf1, BRF1, RNA polymerase III transcription initiation factor subunit, transcription initiation factor IIB, general transcription factor IIB, cilia and flagella associated protein 20, transcription factor IIb, putative, transcription factor IIB, transcription factor IIb, Transcription factor iib, putative, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit, CG31256 gene product from transcript CG31256-RA, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit L homeolog, BRF1, gtf2b, GTF2B, cfap20, PFE0415w, Tb09.160.4220, PVX_097980, CpipJ_CPIJ011738, CpipJ_CPIJ014799, CpipJ_CPIJ018710, PKH_102450, Brf1, Brf, brf1.L
    Background
    RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in alignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: B-related factor 1, BRF, GTF3B, RNA polymerase III subunit 2, TAF3B2, TAF3C, TATA box-binding protein-associated factor, Transcription factor IIIB 90 kDa subunit
    Molecular Weight
    approx. 74 kDa
    Gene ID
    2972
    NCBI Accession
    NP_001510
    UniProt
    Q92994
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