Expected / apparent Molecular Weight of the Antigene: 25-38 kDa for A. thaliana Antibody detection limit is 7 pg, 20 fmol, linear range of logit/logplot is 0.023-15pmol,midrange (B/Bo=50%) is 120 pg, 342 fmol .
Purification
serum
Immunogen
synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490) and sAPX (At1g08390) protein sequences, coupled to BSA. Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At1g09640) and pAPX (At1g35000)
Recommended Dilution: 1 : 2000 with standard ECL (WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL of sterile water
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).
Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." in: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, (2008) (PubMed).
Kangasjaervi, Lepistoe, Haennikaeinen, Piippo, Luomala, Aro, Rintamaeki: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." in: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, (2008) (PubMed).
AGI Code: At1g77490 APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).