CD34 antibody (CD34) (FITC) Primary Antibody
CD34 Reactivity: Human, Non-Human Primate FACS, Func, IHC (f), IHC (p), IP, WB Host: Mouse Monoclonal QBEnd-10
Catalog No. ABIN343723
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- Human, Non-Human Primate
- This CD34 antibody is conjugated to FITC
- Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
- The antibody QBEnd-10 reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
HLDA V., WS Code BP BP275
HLDA V., WS Code E E038
HLDA V., WS Code M MA065
HLDA V., WS Code M MR09
- No Cross-Reactivity
- Rat (Rattus), Cow (Bovine), Sheep (Ovine), Dog (Canine)
- Cross-Reactivity (Details)
- Human, Non-Human Primates
- Human endothelial vesicles
- Application Notes
- The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
- For Research Use only
- No reconstitution is necessary.
- The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
Avoid prolonged exposure to light.
- 4 °C
- Storage Comment
- Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, 1994 (PubMed).
Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, 1994 (PubMed).
Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, 1992 (PubMed).
Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, 1992 (PubMed).
Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, 1991 (PubMed).
Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, 1990 (PubMed).
- Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, 1994 (PubMed).
- Alternative Name
- CD34 (CD34 Antibody Abstract)
- AU040960, CD34 molecule, CD34 antigen, CD34, Cd34
- CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
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