FAP antibody (Internal Region)

Catalog No. ABIN349584

Supplier Product No.: 600-401-a73

Product Details anti-FAP Antibody

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-a73

Supplier: Rockland

Purpose: Fibroblast Activation Protein Antibody

Cross-Reactivity (Details): This antibody is known to react with mouse FAP protein.

Characteristics: Synonyms: rabbit anti-Fap antibody, Fibroblast activation protein alpha, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Integral membrane serine protease antibody, FAPA antibody, Gelatine degradation protease FAP, Surface-expressed protease, SIMP, Seprase, APCE

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 to 1:2,000

ELISA Dilution: 1:10,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.81 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: Fap (FAP Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4.  Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Gene ID: 14089, 6679749

UniProt: P97321

Pathways: Tube Formation