SMAD1 antibody (pSer206)

Catalog No. ABIN349630

Supplier Product No.: 600-401-b29

Product Details anti-SMAD1 Antibody

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Binding Specificity: pSer206

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-b29

Supplier: Rockland

Purpose: SMAD1 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue.

Characteristics: Synonyms: rabbit anti-SMAD1 pS206 antibody, SMAD-1, SMAD 1, mothers against decapentaplegic homolog 1 antibody, MAD homolog 1, Mothers against DPP homolog 1, SMAD family member 1, MADH1, MADH 1, Transforming growth factor-beta-signaling protein 1, BSP-1, JV4-1

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to phosphorylated SMAD1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This antibody is specific for the phosphorylated pS206 of SMAD1. Stimulation of EGF, TGFbeta BMP2, and 0.5M NaCl are recommended for 1 hour. This antibody is useful in ChIP and ChIPSeq.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: 2 μg

ELISA Dilution: 1:25,000-1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.31 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Background: Background: SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development