Mitochondria antibody

Catalog No. ABIN361348

Product Details

Target: Mitochondria

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: Specific for human mitochondria in all cell types. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.

Cross-Reactivity: Human

Purification: Protein A purified

Immunogen: Human cell homogenate

Clone: 113-1

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IHC: 1:800 IF: 1:800 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in PBS

Storage: -20 °C

References

Bussard, Smith: "Human breast cancer cells are redirected to mammary epithelial cells upon interaction with the regenerating mammary gland microenvironment in-vivo." in: PLoS ONE, Vol. 7, Issue 11, pp. e49221, (2012) (PubMed).

Target Details

Target: Mitochondria

Background: Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Molecular Weight: '60 kDa