Mitochondria antibody
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- Target
- Mitochondria
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Characteristics
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
- Purification
- Protein G affinity chromatography
- Immunogen
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.
- Clone
- SPM198
- Isotype
- IgG1 kappa
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- Application Notes
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Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the anti-Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Mitochondria
- Background
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
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