Mitochondria antibody

Catalog No. ABIN6655078

Product Details

Target: Mitochondria

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Purification: Anti-Mitochondria antibody is specific for human mitochondria in all cell types. The antibody is a protein A purification from concentrated clarified cell culture supernate. The antibody reacts specifically with human cells, including neurons and embryonic stem cells. The antibody does not crossreact with mitochondria from mouse or rat.

Immunogen:

Immunogen: Anti-Mitochondria Monoclonal Antibody was produced by repeated immunizations with a human cell homogenate.

Immunogen Type: Other

Clone: 113-1

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:800

Application Note: Anti-Mitochondria antibody is suitable for use in Western Blotting, IHC and IF. Expect ~60 kDa in WB. Anti-Anti-Mitochondria antibody works on acetone fixed and 2 % paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2 % paraformaldehyde fixed cryostat sections. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:10,000 - 1:40,000

Western Blot Dilution: 1:200 - 1:2,000

IF Microscopy Dilution: 1:100 - 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details

Target: Mitochondria

Background:

Synonyms: mouse anti-Mitochondria Antibody, Human cell marker antibody, xenographic model research antibodies, anti-mitochondria antibody produced in mouse

Background: Mitochondria antibody detects human Mitochondria. Mitochonria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation. In addition, mitochondria have been shown to play a role in apoptosis. This antibody is an excellent marker for human cells in xenographic model research.