Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody

Details for Product No. ABIN361462
Target
Binding Specificity
pThr34
30
20
15
9
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat
124
109
95
14
11
11
8
5
3
3
3
1
1
1
1
Host
Rabbit
122
7
1
1
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
5
5
5
5
3
2
2
2
2
2
2
1
Application
Western Blotting (WB)
89
44
41
27
23
16
7
6
5
4
4
1
1
Options
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr34. Immunolabeling is blocked by (-phosphatase treatment.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Predicted Reactivity bovine, canine, chicken, human, non-human primates
Purification Antigen Affinity Purified from Pooled Serum
Target
Alternative Name PPP1R1B (PPP1R1B Antibody Abstract)
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-Phospho Thr34 DARPP-32 Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with (-Ptase.
Molecular Weight '32 kDa
Gene ID 360616
UniProt Q6J4I0
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Buffer 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage -20 °C
Images
Image no. 1 for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody (ABIN361462) Western blots of rat caudate lysate showing specific immunolabeling of the ~32k DARPP...
Product cited in: DelGuidice, Latapy, Rampino, Khlghatyan, Lemasson, Gelao, Quarto, Rizzo, Barbeau, Lamarre, Bertolino, Blasi, Beaulieu: "FXR1P is a GSK3β substrate regulating mood and emotion processing." in: Proceedings of the National Academy of Sciences of the United States of America, 2015 (PubMed).

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, 2012 (PubMed).

Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, 2011 (PubMed).

Daigle, Wetsel, Caron: "Opposite function of dopamine D1 and N-methyl-D-aspartate receptors in striatal cannabinoid-mediated signaling." in: The European journal of neuroscience, Vol. 34, Issue 9, pp. 1378-89, 2011 (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, 2010 (PubMed).

Santini, Sgambato-Faure, Li, Savasta, Dovero, Fisone, Bezard: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, 2010 (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, 2008 (PubMed).

Beaulieu, Sotnikova, Gainetdinov, Caron: "Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice." in: The Journal of biological chemistry, Vol. 281, Issue 43, pp. 32072-80, 2006 (PubMed).