Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody

Details for Product No. ABIN361462
Antigen
  • darpp32
  • darpp-32
  • Darpp-32
  • Darpp32
  • DARPP-32
  • DARPP32
  • AU040756
  • protein phosphatase 1 regulatory inhibitor subunit 1B
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog
  • ppp1r1b
  • PPP1R1B
  • Ppp1r1b
  • ppp1r1b.S
Epitope
pThr34
47
46
15
14
8
5
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
205
151
141
16
12
12
11
6
3
3
3
1
1
1
1
Host
Rabbit
208
14
2
2
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
7
7
7
7
7
3
2
2
2
2
2
2
2
Application
Western Blotting (WB)
148
74
67
30
28
27
6
6
6
6
2
1
Options
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr34. Immunolabeling is blocked by (-phosphatase treatment.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Predicted Reactivity bovine, canine, chicken, human, non-human primates
Purification Antigen Affinity Purified from Pooled Serum
Antigen
Alternative Name PPP1R1B (PPP1R1B Antibody Abstract)
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-Phospho Thr34 DARPP-32 Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with (-Ptase.
Molecular Weight '32 kDa
Gene ID 360616
UniProt Q6J4I0
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Buffer 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody (ABIN361462) Western blots of rat caudate lysate showing specific immunolabeling of the ~32k DARPP...
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody (ABIN361462) Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DA...
Product cited in: DelGuidice, Latapy, Rampino, Khlghatyan, Lemasson, Gelao, Quarto, Rizzo, Barbeau, Lamarre, Bertolino, Blasi, Beaulieu: "FXR1P is a GSK3β substrate regulating mood and emotion processing." in: Proceedings of the National Academy of Sciences of the United States of America, 2015 (PubMed).

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, 2012 (PubMed).

Marazziti, Di Pietro, Mandillo, Golini, Matteoni, Tocchini-Valentini: "Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, DeltaFosB expression, and conditioned place preference to amphetamine and cocaine." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 2071-81, 2011 (PubMed).

Daigle, Wetsel, Caron: "Opposite function of dopamine D1 and N-methyl-D-aspartate receptors in striatal cannabinoid-mediated signaling." in: The European journal of neuroscience, Vol. 34, Issue 9, pp. 1378-89, 2011 (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, 2010 (PubMed).

Santini, Sgambato-Faure, Li, Savasta, Dovero, Fisone, Bezard: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, 2010 (PubMed).

Niculescu, Perrine, Miller, Ehrlich, Unterwald: "Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 5, pp. 1198-207, 2008 (PubMed).

Beaulieu, Sotnikova, Gainetdinov, Caron: "Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice." in: The Journal of biological chemistry, Vol. 281, Issue 43, pp. 32072-80, 2006 (PubMed).

Background publications Lindskog, Svenningsson, Pozzi, Kim, Fienberg, Bibb, Fredholm, Nairn, Greengard, Fisone: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Maldve, Zhang, Ferrani-Kile, Schreiber, Lippmann, Snyder, Fienberg, Leslie, Gonzales, Morrisett: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Svenningsson, Tzavara, Witkin, Fienberg, Nomikos, Greengard: "Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3182-7, 2002 (PubMed).

Bibb, Snyder, Nishi, Yan, Meijer, Fienberg, Tsai, Kwon, Girault, Czernik, Huganir, Hemmings, Nairn, Greengard: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Fienberg, Hiroi, Mermelstein, Song, Snyder, Nishi, Cheramy, OCallaghan, Miller, Cole, Corbett, Haile, Cooper, Onn, Grace, Ouimet, White, Hyman, Surmeier, Girault, Nestler, Greengard: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

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