Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

IRF8 antibody (Interferon Regulatory Factor 8) (C-Term) Primary Antibody

IRF8 Reactivity: Human, Mouse, Rat, Cow WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN374256
$537.90
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Available
  • Target
    IRF8
    Binding Specificity
    • 18
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 46
    • 21
    • 14
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow
    Host
    • 44
    • 7
    • 2
    Goat
    Clonality
    • 51
    • 2
    Polyclonal
    Conjugate
    • 37
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IRF8 antibody is un-conjugated
    Application
    • 41
    • 23
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    C-QRSFFRENQQ ITV
    Specificity
    This antibody detects IRF8 / ICSBP1 at C-term.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from C Terminus of the protein sequence according to NP_002154.1 Genename: IRF8
  • Application Notes
    Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 45-50 kDa band seen in K562 lysate.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    IRF8
    Alternative Name
    IRF8 / ICSBP1 (IRF8 Antibody Abstract)
    Synonyms
    zgc:92252, IRF8, MGC140338, DKFZp469B0320, H-ICSBP, ICSBP, ICSBP1, IRF-8, icsbp1, AI893568, Icsbp1, Myls, interferon regulatory factor 8, interferon regulatory factor 8 L homeolog, irf8, IRF8, irf8.L, Irf8
    Background
    Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl 2 expression and thus may play a role in chronic myelogenous leukemia.Synonyms: IRF-8, Interferon consensus sequence-binding protein, Interferon regulatory factor 8
    Gene ID
    3394
    NCBI Accession
    NP_002154
    UniProt
    Q02556
    Pathways
    Cellular Response to Molecule of Bacterial Origin
You are here:
help Support