KPNA1 antibody (Karyopherin alpha 1 (Importin alpha 5)) (N-Term) Primary Antibody
- Human, Mouse, Rat, Cow, Dog, Pig
- This KPNA1 antibody is un-conjugated
- Western Blotting (WB)
- TTPGKENFRL KSY-C
- This antibody detects Importin subunit alpha-1 at N-term.
- Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):Human
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (N-Term) antibody Primary Antibody
KPNA1 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated
- Application Notes
- Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).
- For Research Use only
- 0.5 mg/mL
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
- Alternative Name
- KPNA1 / Importin alpha-1 (KPNA1 Antibody Abstract)
- IPOA5, NPI-1, RCH2, SRP1, AW494490, NPI1, Rch2, mSRP1, si:ch211-161h7.7, karyopherin subunit alpha 1, importin alpha-1 subunit, karyopherin (importin) alpha 1, karyopherin alpha 1 (importin alpha 5), KPNA1, Kpna1, EHI_124900, kpna1
- Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta
- Gene ID
- NCBI Accession
- M Phase, Protein targeting to Nucleus