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KPNA1 antibody (Karyopherin alpha 1 (Importin alpha 5)) (N-Term) Primary Antibody

KPNA1 Reactivity: Human, Mouse, Rat, Cow, Dog, Pig WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN374391
$537.90
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0.1 mg
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  • Target
    KPNA1
    Binding Specificity
    • 14
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 47
    • 17
    • 16
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat, Cow, Dog, Pig
    Host
    • 29
    • 10
    • 7
    • 1
    Goat
    Clonality
    • 40
    • 7
    Polyclonal
    Conjugate
    • 28
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KPNA1 antibody is un-conjugated
    Application
    • 38
    • 25
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    TTPGKENFRL KSY-C
    Specificity
    This antibody detects Importin subunit alpha-1 at N-term.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
    Species reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1
  • Application Notes
    Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    KPNA1
    Alternative Name
    KPNA1 / Importin alpha-1 (KPNA1 Antibody Abstract)
    Synonyms
    IPOA5, NPI-1, RCH2, SRP1, AW494490, NPI1, Rch2, mSRP1, si:ch211-161h7.7, karyopherin subunit alpha 1, importin alpha-1 subunit, karyopherin (importin) alpha 1, karyopherin alpha 1 (importin alpha 5), KPNA1, Kpna1, EHI_124900, kpna1
    Background
    Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta
    Gene ID
    3836
    NCBI Accession
    NP_002255
    UniProt
    P52294
    Pathways
    M Phase, Protein targeting to Nucleus
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