GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term) Primary Antibody
- Human, Mouse, Rat, Dog
- This GAPDH antibody is un-conjugated
- Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- C-HQVVSSDFNS DT
- This antibody is specific to GAPDH at C-Term. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.
- Cross-Reactivity (Details)
Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Peptide with sequence from the C-Terminus of the protein sequence according to NP_002037.2.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Center) antibody Primary Antibody
GAPDH Reactivity: Human, Mouse, Rat, Dog, Drosophila melanogaster, E. coli ICC, IF, IHC (rs), IP, IHC (p), WB Host: Rabbit Polyclonal unconjugatedanti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody Primary Antibody
GAPDH Reactivity: Human, Mouse, Rat, Chicken, Cow, Goat, Monkey, Pig, Zebrafish (Danio rerio) ELISA, ICC, IF, IHC, WB Host: Rabbit Polyclonal unconjugatedanti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (N-Term) antibody Primary Antibody
GAPDH Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep IHC, WB Host: Rabbit Polyclonal unconjugated
- Application Notes
- Peptide ELISA: Detection Limit: 1/2000Western Blot: 0.01-0.03 μg/mL. A approx. 35 kDa band is observed in lysates of Human Tonsil,MouseSpleen, and cell lines HeLa, A431 and 293. Immunohistochemistry In Paraffin Embedded Tonsil: 0.3 μg/mL. Staining of cells ingerminal centre, mantle zone, interfollicular area, epithelium and endothelium is
- For Research Use only
- 0.5 mg/mL
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Farris, Pifer, Zheng, Gottlieb, Denvir, Frisch: "Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis." in: Molecular cancer research : MCR, Vol. 14, Issue 6, pp. 528-38, 2017 (PubMed).
- Alternative Name
- GAPDH (GAPDH Antibody Abstract)
- G3PD, GAPD, Gapd, BEST:GH12586, CG12055, Dmel\\CG12055, GA3PDH, GADPH, GAP, GAPDH, GAPDH I, GAPDH-1, GAPDH1, GAPDHI, Gapdh, Gapdh-1, Gapdh43E, gadph, gapdh, gapdh-1, gh12586, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, g3pd, G3PDH, glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase S homeolog, glyceraldehyde-3-phosphate dehydrogenase, type I, olfactory receptor 8K3, GAPDH, Gapdh, Gapdh1, gapdh, gapdh.S, gapDH, LOC100404960, LOC614985
- Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
- Molecular Weight
- 36.1 kDa (Calculated)
- Gene ID
- NCBI Accession